Cell Biology Antibodies 3
Anti-CD248 Antibody (CAB12703)
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- SKU:
- CAB12703
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CD248 Antibody |
| Antibody SKU: | CAB12703 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-150 of human CD248 (NP_065137.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A375, Mouse spleen, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-150 of human CD248 (NP_065137.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QDPW AAEP RAAC GPSS CYAL FPRR RTFL EAWR ACRE LGGD LATP RTPE EAQR VDSL VGAG PASR LLWI GLQR QARQ CQLQ RPLR GFTW TTGD QDTA FTNW AQPA SGGP CPAQ RCVA LEAS GEHR WLEG SCTL A |
| Gene ID: | 57124 |
| Uniprot: | Q9HCU0 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 46kDa/80kDa |
| Observed MW: | 71-81kDa |
| Synonyms: | CD248, CD164L1, TEM1 |
| Background: |
| UniProt Protein Function: | CD248: May play a role in tumor angiogenesis. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 11q13 Cellular Component: proteinaceous extracellular matrix; cytoplasm; integral to membrane Molecular Function:extracellular matrix binding; calcium ion binding; carbohydrate binding Biological Process: cell migration; positive regulation of cell proliferation; anatomical structure regression; lymph node development |
| UniProt Code: | Q9HCU0 |
| NCBI GenInfo Identifier: | 74752810 |
| NCBI Gene ID: | 57124 |
| NCBI Accession: | Q9HCU0.1 |
| UniProt Related Accession: | Q9HCU0 |
| Molecular Weight: | |
| NCBI Full Name: | Endosialin |
| NCBI Synonym Full Names: | CD248 molecule |
| NCBI Official Symbol: | CD248 |
| NCBI Official Synonym Symbols: | TEM1; CD164L1 |
| NCBI Protein Information: | endosialin |
| UniProt Protein Name: | Endosialin |
| UniProt Synonym Protein Names: | Tumor endothelial marker 1; CD_antigen: CD248 |
| Protein Family: | Endosialin |
| UniProt Gene Name: | CD248 |
| UniProt Entry Name: | CD248_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD248 antibody (CAB12703) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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