Cell Biology Antibodies 8

Anti-CD18 Antibody (CAB2173)

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SKU:
CAB2173
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CD18 Antibody
Antibody SKU:CAB2173
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-937, Mouse thymus, Mouse lung, Rat thymus
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 70-170 of human CD18 (NP_000202.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRGC AADD IMDP TSLA ETQE DHNG GQKQ LSPQ KVTL YLRP GQAA AFNV TFRR AKGY PIDL YYLM DLSY SMLD DLRN VKKL GGDL LRAL NEIT ESGR IGFG S
Gene ID:3689
Uniprot:P05107
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:84kDa
Observed MW:100kDa
Synonyms:CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, ITGB2
Background:This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, misc.; Cell surface; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function:protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; toll-like receptor 4 signaling pathway; regulation of peptidyl-tyrosine phosphorylation; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; innate immune response; endothelial cell migration; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I

NCBI Summary:This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:P05107
NCBI GenInfo Identifier:124056465
NCBI Gene ID:3689
NCBI Accession:P05107.2
UniProt Secondary Accession:P05107,Q16418, Q53HS5, Q9UD72, B3KTS8, D3DSM1,
UniProt Related Accession:P05107
Molecular Weight:769
NCBI Full Name:Integrin beta-2
NCBI Synonym Full Names:integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
NCBI Official Symbol:ITGB2  
NCBI Official Synonym Symbols:LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1  
NCBI Protein Information:integrin beta-2; integrin beta chain, beta 2; complement receptor C3 beta-subunit; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor)
UniProt Protein Name:Integrin beta-2
UniProt Synonym Protein Names:Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD_antigen: CD18
Protein Family:Integrin
UniProt Gene Name:ITGB2  
UniProt Entry Name:ITB2_HUMAN
Anti-CD18 Antibody (CAB2173)Western blot analysis of extracts of various cell lines, using CD18 antibody (CAB2173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00021). Exposure time: 30s.
Anti-CD18 Antibody (CAB2173)Immunohistochemistry of paraffin-embedded Rat lung using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens).
Anti-CD18 Antibody (CAB2173)Immunohistochemistry of paraffin-embedded Rat spleen using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens).
Anti-CD18 Antibody (CAB2173)Immunohistochemistry of paraffin-embedded Mouse spleen using CD18 antibody (CAB2173) at dilution of 1:100 (40x lens).
Anti-CD18 Antibody (CAB2173)Immunofluorescence analysis of RAW264.7 cells using CD18 antibody (CAB2173) at dilution of 1:100. Blue: DAPI for nuclear staining.
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