Description
Antibody Name: | Anti-CD168/RHAMM Antibody (CAB2540) |
Antibody SKU: | CAB2540 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 341-440 of human CD168/RHAMM (NP_001136028.1). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, Mouse lung, Mouse spleen, Mouse small intestine, Mouse thymus, Rat testis |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 341-440 of human CD168/RHAMM (NP_001136028.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | LQID SLLQ QEKE LSSS LHQK LCSF QEEM VKEK NLFE EELK QTLD ELDK LQQK EEQA ERLV KQLE EEAK SRAE ELKL LEEK LKGK EAEL EKSS AAHT QATL |
Cellular Location: | Cell surface, Cytoplasm |
Calculated MW: | 74kDa/82kDa/84kDa |
Observed MW: | 90kDa |
Synonyms: | HMMR, CD168, IHABP, RHAMM |
Background: | The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
Western blot analysis of extracts of various cell lines, using CD168/RHAMM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |