Antibody Name:	Anti-CD133 Antibody [KO Validated]
Antibody SKU:	CAB12711
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:1000 - 1:3000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 22Rv1, Mouse kidney, Mouse pancreas, Mouse lung, Rat kidney, HCT116

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FEHY LQWI EFSI SEKV ASCK PVAT ALDT AVDV FLCS YIID PLNL FWFG IGKA TVFL LPAL IFAV KLAK YYRR MDSE DVYD DVET IPMK NMEN GNNG YHKD H
Gene ID:	8842
Uniprot:	O43490
Cellular Location:	Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment
Calculated MW:	92kDa/93kDa/94kDa/96kDa/97kDa
Observed MW:	115kDa

Synonyms:

PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4

Background:

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell surface; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: stereocilium; extracellular space; cell surface; photoreceptor outer segment; microvillus membrane; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; vesicle; brush border Molecular Function:actinin binding; protein binding; cadherin binding Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12
NCBI Summary:	This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	O43490
NCBI GenInfo Identifier:	13124442
NCBI Gene ID:	8842
NCBI Accession:	O43490.1
UniProt Secondary Accession:	O43490,Q6SV49, Q6SV50, Q6SV51, Q6SV52, Q6SV53, Q96EN6
UniProt Related Accession:	O43490
Molecular Weight:	97kDa
NCBI Full Name:	Prominin-1
NCBI Synonym Full Names:	prominin 1
NCBI Official Symbol:	PROM1
NCBI Official Synonym Symbols:	RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
NCBI Protein Information:	prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen
UniProt Protein Name:	Prominin-1
UniProt Synonym Protein Names:	Antigen AC133; Prominin-like protein 1; CD_antigen: CD133
Protein Family:	Prominin
UniProt Gene Name:	PROM1
UniProt Entry Name:	PROM1_HUMAN

	Western blot analysis of extracts of various cell lines, using CD133 antibody (CAB12711) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts from normal (control) and CD133 Rabbit pAb knockout (KO) HCT116 cells, using CD133 Rabbit pAb antibody (CAB12711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH/3T3 cells using CD133 antibody (CAB12711) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CD133 antibody (CAB12711) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 antibody (CAB12711). Western blot was performed from the immunoprecipitate using CD133 antibody (CAB12711) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-CD133 Antibody (CAB0219)	Human Prominin-1 / CD133 ELISA Kit
Anti-PROM1 Antibody (CAB0510)	Human Prominin-1 (PROM1) ELISA Kit
PROM1 Antibody (PACO01905)
PROM1 Antibody (PACO11492)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)