Cell Biology Antibodies 1

Anti-CD133 Antibody (CAB0219)

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SKU:
CAB0219
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CD133 Antibody
Antibody SKU:CAB0219
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 170-430 of human CD133 (NP_001139320.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, Mouse testis, Mouse kidney, Mouse pancreas, Rat lung, Rat kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 170-430 of human CD133 (NP_001139320.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ANHQ VRTR IKRS RKLA DSNF KDLR TLLN ETPE QIKY ILAQ YNTT KDKA FTDL NSIN SVLG GGIL DRLR PNII PVLD EIKS MATA IKET KEAL ENMN STLK SLHQ QSTQ LSSS LTSV KTSL RSSL NDPL CLVH PSSE TCNS IRLS LSQL NSNP ELRQ LPPV DAEL DNVN NVLR TDLD GLVQ QGYQ SLND IPDR VQRQ TTTV VAGI KRVL NSIG SDID NVTQ RLPI QDIL SAFS VYVN NTES YIHR NLPT LEEY DSYW WLGG L
Gene ID:8842
Uniprot:O43490
Cellular Location:Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment
Calculated MW:92kDa/93kDa/94kDa/96kDa/97kDa
Observed MW:97kDa/125kDa
Synonyms:PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Background:This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: stereocilium; extracellular space; cell surface; photoreceptor outer segment; microvillus membrane; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; vesicle; brush border

Molecular Function:actinin binding; protein binding; cadherin binding

Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye

Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

NCBI Summary:This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:O43490
NCBI GenInfo Identifier:13124442
NCBI Gene ID:8842
NCBI Accession:O43490.1
UniProt Secondary Accession:O43490,Q6SV49, Q6SV50, Q6SV51, Q6SV52, Q6SV53, Q96EN6
UniProt Related Accession:O43490
Molecular Weight:97kDa
NCBI Full Name:Prominin-1
NCBI Synonym Full Names:prominin 1
NCBI Official Symbol:PROM1  
NCBI Official Synonym Symbols:RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061  
NCBI Protein Information:prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen
UniProt Protein Name:Prominin-1
UniProt Synonym Protein Names:Antigen AC133; Prominin-like protein 1; CD_antigen: CD133
Protein Family:Prominin
UniProt Gene Name:PROM1  
UniProt Entry Name:PROM1_HUMAN
Anti-CD133 Antibody (CAB0219)Western blot analysis of extracts of various cell lines, using CD133 antibody (CAB0219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-CD133 Antibody (CAB0219)Immunohistochemistry of paraffin-embedded human prostate using CD133 antibody (CAB0219) at dilution of 1:100 (40x lens).
Anti-CD133 Antibody (CAB0219)Immunohistochemistry of paraffin-embedded human lung using CD133 antibody (CAB0219) at dilution of 1:100 (40x lens).
Anti-CD133 Antibody (CAB0219)Immunofluorescence analysis of C6 cells using CD133 antibody (CAB0219) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CD133 Antibody (CAB0219)Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 antibody (CAB0219). Western blot was performed from the immunoprecipitate using CD133 antibody (CAB0219) at a dilition of 1:1000.
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Anti-CD133 Antibody (CAB0818)

OverviewProduct Name:CD133 Rabbit mAbProduct Code:CAB0818Size:20uL, 50uL, 100uLSynonyms:PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PRO