Cell Biology Antibodies 2

Anti-CCT7 Antibody (CAB12146)

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SKU:
CAB12146
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CCT7 Antibody
Antibody SKU:CAB12146
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-500 of human CCT7 (NP_006420.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-431, HT-29, HeLa, NIH/3T3, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-500 of human CCT7 (NP_006420.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CAGR VPEE DLKR TMMA CGGS IQTS VNAL SADV LGRC QVFE ETQI GGER YNFF TGCP KAKT CTFI LRGG AEQF MEET ERSL HDAI MIVR RAIK NDSV VAGG GAIE MELS KYLR DYSR TIPG KQQL LIGA YAKA LEII PRQL CDNA GFDA TNIL NKLR ARHA QGGT WYGV DINN EDIA DNFE AFVW EPAM VRI
Gene ID:10574
Uniprot:Q99832
Cellular Location:Cytoplasm
Calculated MW:37kDa/50kDa/54kDa/59kDa
Observed MW:55kDa
Synonyms:CCT7, CCTETA, CCTH, NIP7-1, TCP1ETA
Background:This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.
UniProt Protein Function:CCT7: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Chaperone

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: chaperonin-containing T-complex; cytoplasm; cytosol; microtubule

Molecular Function:protein binding; unfolded protein binding

Biological Process: 'de novo' protein folding; positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization

NCBI Summary:This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009]
UniProt Code:Q99832
NCBI GenInfo Identifier:3041738
NCBI Gene ID:10574
NCBI Accession:Q99832.2
UniProt Secondary Accession:Q99832,O14871, Q6FI26, A8K7E6, A8MWI8, B7WNW9, B7Z4T9 B7Z4Z7,
UniProt Related Accession:Q99832
Molecular Weight:59kDa
NCBI Full Name:T-complex protein 1 subunit eta
NCBI Synonym Full Names:chaperonin containing TCP1 subunit 7
NCBI Official Symbol:CCT7  
NCBI Official Synonym Symbols:CCTH; CCTETA; NIP7-1; TCP1ETA  
NCBI Protein Information:T-complex protein 1 subunit eta
UniProt Protein Name:T-complex protein 1 subunit eta
UniProt Synonym Protein Names:CCT-eta; HIV-1 Nef-interacting protein
Protein Family:Probable T-complex protein
UniProt Gene Name:CCT7  
Anti-CCT7 Antibody (CAB12146)Western blot analysis of extracts of various cell lines, using CCT7 antibody (CAB12146) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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