Antibody Name:	Anti-CCT6B Antibody
Antibody SKU:	CAB14615
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human CCT6B (NP_006575.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human CCT6B (NP_006575.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEGF EAAK IKAL EVLE EVKV TKEM KRKI LLDV ARTS LQTK VHAE LADV LTEV VVDS VLAV RRPG YPID LFMV EIME MKHK LGTD TKLI QGLV LDHG ARHP DMKK RVED AFIL ICNV SLEY EKTE VNSG FFYK TAEE KEKL VKAE RKFI EDRV QKII DLKD KVCA QSNK GFVV INQK GIDP FSLD SLAK HGIV ALRR AKRR NMER LSLA CGGM AVNS FEDL T
Gene ID:	10693
Uniprot:	Q92526
Cellular Location:	Cytoplasm
Calculated MW:	53kDa/57kDa
Observed MW:	58kDa

Synonyms:

CCT6B, CCT-zeta-2, CCTZ-2, Cctz2, TCP-1-zeta-2, TSA303

Background:

This gene encodes a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CCT-zeta-2: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 17q12 Cellular Component: chaperonin-containing T-complex Molecular Function:unfolded protein binding; protein transporter activity; ATP binding Biological Process: protein transport; protein folding; spermatogenesis; chaperone-mediated protein complex assembly
NCBI Summary:	This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	Q92526
NCBI GenInfo Identifier:	119600607
NCBI Gene ID:	10693
NCBI Accession:	EAW80201.1
UniProt Secondary Accession:	Q92526,Q8TC34, B4DX20, B4DYB0,
UniProt Related Accession:	Q92526
Molecular Weight:	57,821 Da
NCBI Full Name:	chaperonin containing TCP1, subunit 6B (zeta 2)
NCBI Synonym Full Names:	chaperonin containing TCP1, subunit 6B (zeta 2)
NCBI Official Symbol:	CCT6B
NCBI Official Synonym Symbols:	Cctz2; CCTZ-2; TSA303; CCT-zeta-2; TCP-1-zeta-2
NCBI Protein Information:	T-complex protein 1 subunit zeta-2; testis-specific Tcp20; testis-specific protein TSA303; T-complex protein 1, zeta-2 subunit
UniProt Protein Name:	T-complex protein 1 subunit zeta-2
UniProt Synonym Protein Names:	CCT-zeta-2; CCT-zeta-like; TCP-1-zeta-like; Testis-specific Tcp20; Testis-specific protein TSA303
Protein Family:	Choline-phosphate cytidylyltransferase
UniProt Gene Name:	CCT6B
UniProt Entry Name:	TCPW_HUMAN

	Western blot analysis of extracts of various cell lines, using CCT6B antibody (CAB14615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of HeLa cells using CCT6B antibody (CAB14615) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using CCT6B antibody (CAB14615) at dilution of 1:100. Blue: DAPI for nuclear staining.