Cell Biology Antibodies 10
Anti-CCT4 Antibody (CAB6548)
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- SKU:
- CAB6548
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CCT4 Antibody |
| Antibody SKU: | CAB6548 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, THP-1, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PTII SESF QKAL EKGI EILT DMSR PVEL SDRE TLLN SATT SLNS KVVS QYSS LLSP MSVN AVMK VIDP ATAT SVDL RDIK IVKK LGGT IDDC ELVE GLVL TQKV SNSG ITRV EKAK IGLI QFCL SAPK TDMD NQIV VSDY AQMD RVLR EERA YILN LVKQ IKKT GCNV LLIQ KSIL RDAL SDLA LHFL NKMK IMVI KDIE REDI EFIC KTIG TKPV AHID QFTA DMLG SAEL AEEV NLNG SGKL LKIT GCAS PGKT VTIV V |
| Gene ID: | 10575 |
| Uniprot: | P50991 |
| Cellular Location: | Cytoplasm, Melanosome, centrosome, cilium basal body, cytoskeleton, microtubule organizing center |
| Calculated MW: | 54kDa/57kDa |
| Observed MW: | 60kDa |
| Synonyms: | CCT4, CCT-DELTA, Cctd, SRB |
| Background: |
| UniProt Protein Function: | CCT4: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. |
| UniProt Protein Details: | Protein type:Chaperone Chromosomal Location of Human Ortholog: 2p15 Cellular Component: centrosome; chaperonin-containing T-complex; cytoplasm; cytosol; microtubule; nucleoplasm Molecular Function:protein binding Biological Process: positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein folding; protein stabilization |
| NCBI Summary: | The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | P50991 |
| NCBI GenInfo Identifier: | 52001478 |
| NCBI Gene ID: | 10575 |
| NCBI Accession: | P50991.4 |
| UniProt Secondary Accession: | P50991,O14870, Q53QP9, Q96C51, B2R6I3, B7Z8B1, F5H5W3 |
| UniProt Related Accession: | P50991 |
| Molecular Weight: | 54,720 Da |
| NCBI Full Name: | T-complex protein 1 subunit delta |
| NCBI Synonym Full Names: | chaperonin containing TCP1 subunit 4 |
| NCBI Official Symbol: | CCT4 |
| NCBI Official Synonym Symbols: | SRB; Cctd; CCT-DELTA |
| NCBI Protein Information: | T-complex protein 1 subunit delta |
| UniProt Protein Name: | T-complex protein 1 subunit delta |
| UniProt Synonym Protein Names: | CCT-delta; Stimulator of TAR RNA-binding |
| Protein Family: | T-complex protein |
| UniProt Gene Name: | CCT4 |
| UniProt Entry Name: | TCPD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CCT4 antibody (CAB6548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat stomach using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using CCT4 antibody (CAB6548) at dilution of 1:100 (40x lens). |
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