Cell Biology Antibodies 10

Anti-CCT3 Antibody (CAB6547)

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SKU:
CAB6547
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CCT3 Antibody
Antibody SKU:CAB6547
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, 293T, HepG2, Mouse brain, Mouse heart, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMGH RPVL VLSQ NTKR ESGR KVQS GNIN AAKT IADI IRTC LGPK SMMK MLLD PMGG IVMT NDGN AILR EIQV QHPA AKSM IEIS RTQD EEVG DGTT SVII LAGE MLSV AEHF LEQQ MHPT VVIS AYRK ALDD MIST LKKI SIPV DISD SDMM LNII NSSI TTKA ISRW SSLA CNIA LDAV KMVQ FEEN GRKE IDIK KYAR VEKI PGGI IEDS CVLR GVMI NKDV THPR MRRY IKNP RIVL LDSS LEYK KGES QTDI EITR EEDF TRIL QMEE EYIQ QLCE DIIQ LKPD VVIT EKGI SDLA
Gene ID:7203
Uniprot:P49368
Cellular Location:Cytoplasm
Calculated MW:56kDa/60kDa
Observed MW:60kDa
Synonyms:CCT3, CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5
Background:The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.
UniProt Protein Function:CCT-gamma: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal; Chaperone

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: chaperonin-containing T-complex; microtubule; cytoskeleton; cytoplasm; plasma membrane; cytosol

Molecular Function:protein binding; unfolded protein binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis

NCBI Summary:The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]
UniProt Code:P49368
NCBI GenInfo Identifier:66774185
NCBI Gene ID:7203
NCBI Accession:P49368.4
UniProt Secondary Accession:P49368,Q5SZY1, Q9BR64, A6NE14,
UniProt Related Accession:P49368
Molecular Weight:545
NCBI Full Name:T-complex protein 1 subunit gamma
NCBI Synonym Full Names:chaperonin containing TCP1, subunit 3 (gamma)
NCBI Official Symbol:CCT3  
NCBI Official Synonym Symbols:CCTG; PIG48; TRIC5; CCT-gamma; TCP-1-gamma  
NCBI Protein Information:T-complex protein 1 subunit gamma; hTRiC5; T-complex protein 1, gamma subunit; TCP1 (t-complex-1) ring complex, polypeptide 5
UniProt Protein Name:T-complex protein 1 subunit gamma
UniProt Synonym Protein Names:CCT-gamma; hTRiC5
Protein Family:Thermosome
UniProt Gene Name:CCT3  
UniProt Entry Name:TCPG_HUMAN
Anti-CCT3 Antibody (CAB6547)Western blot analysis of extracts of various cell lines, using CCT3 antibody (CAB6547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-CCT3 Antibody (CAB6547)Immunohistochemistry of paraffin-embedded mouse heart using CCT3 antibody (CAB6547) at dilution of 1:100 (40x lens).
Anti-CCT3 Antibody (CAB6547)Immunofluorescence analysis of U-2 OS cells using CCT3 antibody (CAB6547) at dilution of 1:100. Blue: DAPI for nuclear staining.
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