Name: Anti-CCDC88A Antibody (CAB16132)
Brand: Cell Biology Antibodies 6
SKU: CAB16132
Availability: OutOfStock

Antibody Name:	Anti-CCDC88A Antibody
Antibody SKU:	CAB16132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1571-1870 of human CCDC88A (NP_001129069.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1571-1870 of human CCDC88A (NP_001129069.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DDSS TGSR VHAS RPAS LDSG RTST SNSN NNAS LHEV KAGA VNNQ SRPQ SHSS GEFS LLHD HEAW SSSG SSPI QYLK RQTR SSPV LQHK ISET LESR HHKI KTGS PGSE VVTL QQFL EESN KLTS VQIK SSSQ ENLL DEVM KSLS VSSD FLGK DKPV SCGL ARSV SGKT PGDF YDRR TTKP EFLR PGPR KTED TYFI SSAG KPTP GTQG KIKL VKES SLSR QSKD SNPY ATLP RASS VIST AEGT TRRT SIHD FLTK DSRL PISV DSPP AAAD SNTT AASN VDKV QESR NSKS RSRE QQSS
Gene ID:	55704
Uniprot:	Q3V6T2
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, cytosol, lamellipodium
Calculated MW:	207kDa/212kDa/215kDa/216kDa
Observed MW:	220kDa

Synonyms:

CCDC88A, APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, girdin

Background:

This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes. AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor; Nuclear receptor Chromosomal Location of Human Ortholog: 7p15 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; signal transducer activity; protein binding; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; Hsp90 protein binding; transcription factor activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of B cell proliferation; circadian regulation of gene expression; positive regulation of transcriptional preinitiation complex assembly; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q3V6T2
NCBI GenInfo Identifier:	208022632
NCBI Gene ID:	55704
NCBI Accession:	NP_001129069
UniProt Related Accession:	Q3V6T2
Molecular Weight:
NCBI Full Name:	girdin isoform 1
NCBI Synonym Full Names:	coiled-coil domain containing 88A
NCBI Official Symbol:	CCDC88A
NCBI Official Synonym Symbols:	APE; GIV; GRDN; PEHO; HkRP1; PEHOL; GIRDIN; KIAA1212
NCBI Protein Information:	girdin
UniProt Protein Name:	Aryl hydrocarbon receptor
UniProt Synonym Protein Names:	Class E basic helix-loop-helix protein 76
Protein Family:	Aldehyde reductase
UniProt Gene Name:	AHR
UniProt Entry Name:	AHR_HUMAN

Western blot analysis of extracts of various cell lines, using CCDC88A antibody (CAB16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Phospho-CCDC88A (S1417) Antibody (PACO04714)

CCDC88A Antibody (PACO04715)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)