Cell Biology Antibodies 6
Anti-CCDC47 Antibody (CAB15871)
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- SKU:
- CAB15871
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CCDC47 Antibody |
| Antibody SKU: | CAB15871 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-483 of human CCDC47 (NP_064583.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, OVCAR3, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-483 of human CCDC47 (NP_064583.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FLKR QDLL NVLA RMMR PVSD QVQI KVTM NDED MDTY VFAV GTRK ALVR LQKE MQDL SEFC SDKP KSGA KYGL PDSL AILS EMGE VTDG MMDT KMVH FLTH YADK IESV HFSD QFSG PKIM QEEG QPLK LPDT KRTL LFTF NVPG SGNT YPKD MEAL LPLM NMVI YSID KAKK FRLN REGK QKAD KNRA RVEE NFLK LTHV QRQE AAQS RREE KKRA EKER IMNE EDPE KQRR LEEA ALRR EQKK LEKK QMKM KQIK VKAM |
| Gene ID: | 57003 |
| Uniprot: | Q96A33 |
| Cellular Location: | Membrane, Single-pass membrane protein |
| Calculated MW: | 55kDa |
| Observed MW: | 60kDa |
| Synonyms: | CCDC47, GK001, MSTP041 |
| Background: |
| UniProt Protein Function: | CCDC47: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 17q23.3 Cellular Component: membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; calcium ion binding Biological Process: calcium ion homeostasis; osteoblast differentiation; ER overload response; post-embryonic development |
| UniProt Code: | Q96A33 |
| NCBI GenInfo Identifier: | 119614685 |
| NCBI Gene ID: | 57003 |
| NCBI Accession: | BC008905 |
| UniProt Related Accession: | Q96A33 |
| Molecular Weight: | Calculated MW: 56 kDaObserved MW: Refer to figures |
| NCBI Full Name: | coiled-coil domain containing 47, isoform CRA_a |
| NCBI Synonym Full Names: | coiled-coil domain containing 47 |
| NCBI Official Symbol: | CCDC47 |
| NCBI Official Synonym Symbols: | THNS; GK001; MSTP041 |
| NCBI Protein Information: | coiled-coil domain-containing protein 47 |
| UniProt Protein Name: | Coiled-coil domain-containing protein 47 |
| Protein Family: | Coiled-coil domain-containing protein |
| UniProt Gene Name: | CCDC47 |
| UniProt Entry Name: | CCD47_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CCDC47 antibody (CAB15871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of C6 cells using CCDC47 antibody (CAB15871) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using CCDC47 antibody (CAB15871) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using CCDC47 antibody (CAB15871) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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