ChIP Antibodies
Anti-CCAR2 Antibody (CAB7126)
- SKU:
- CAB7126
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-CCAR2 Antibody |
| Antibody SKU: | CAB7126 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 654-923 of human CCAR2 (NP_066997.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLRD DGEE EFAG AKLE DSEV RSVA SNQS EMEF SSLQ DMPK ELDP SAVL PLDC LLAF VFFD ANWC GYLH RRDL ERIL LTLG IRLS AEQA KQLV SRVV TQNI CQYR SLQY SRQE GLDG GLPE EVLF GNLD LLPP PGKS TKPG AAPT EHKA LVSH NGSL INVG SLLQ RAEQ QDSG RLYL ENKI HTLE LKLE ESHN RFSA TEVT NKTL AAEM QELR VRLA EAEE TART AERQ KSQL QRLL QELR RRLT PLQL EIQR VVEK ADSW VEKE EPAP SN |
| Gene ID: | 57805 |
| Uniprot: | Q8N163 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 102kDa/103kDa |
| Observed MW: | 100kDa |
| Synonyms: | CCAR2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC |
| Background: |
| UniProt Protein Function: | DBC-1: Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Component of the DBIRD complex. Interacts with ZNF326/ZIRD; the interaction is direct. Interacts (via N-terminus) with SIRT1. Interacts (via N-terminus) with SUV39H1; this interaction abolishes the interaction with SIRT1. Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Apoptosis; RNA-binding; Mitochondrial; Tumor suppressor Chromosomal Location of Human Ortholog: 8p22 Cellular Component: nucleoplasm; spliceosome; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleus Molecular Function:enzyme inhibitor activity; protein binding; enzyme binding Biological Process: positive regulation of apoptosis; RNA splicing; rhythmic process; regulation of protein stability; cell cycle; regulation of circadian rhythm; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of catalytic activity; mitochondrial fragmentation during apoptosis; mRNA processing; regulation of RNA elongation; response to DNA damage stimulus; response to UV |
| UniProt Code: | Q8N163 |
| NCBI GenInfo Identifier: | 85701135 |
| NCBI Gene ID: | 57805 |
| NCBI Accession: | Q8N163.2 |
| UniProt Secondary Accession: | Q8N163,Q6P0Q9, Q8N3G7, Q8N8M1, Q8TF34, Q9H9Q9, Q9HD12 Q9NT55, A6NL03, B2RB79, D3DSR6, |
| UniProt Related Accession: | Q8N163 |
| Molecular Weight: | 923 |
| NCBI Full Name: | Cell cycle and apoptosis regulator protein 2 |
| NCBI Synonym Full Names: | cell cycle and apoptosis regulator 2 |
| NCBI Official Symbol: | CCAR2 |
| NCBI Official Synonym Symbols: | DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967 |
| NCBI Protein Information: | cell cycle and apoptosis regulator protein 2; p30 DBC protein; deleted in breast cancer 1; cell division cycle and apoptosis regulator protein 2 |
| UniProt Protein Name: | Cell cycle and apoptosis regulator protein 2 |
| UniProt Synonym Protein Names: | Cell division cycle and apoptosis regulator protein 2; DBIRD complex subunit KIAA1967; Deleted in breast cancer gene 1 protein; DBC-1; DBC.1; p30 DBC |
| Protein Family: | Cell cycle and apoptosis regulator protein |
| UniProt Gene Name: | CCAR2 |
| UniProt Entry Name: | CCAR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CCAR2 antibody (CAB7126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using CCAR2 antibody (CAB7126) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human rectal cancer using CCAR2 antibody (CAB7126) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using CCAR2 antibody (CAB7126) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using CCAR2 antibody (CAB7126). Blue: DAPI for nuclear staining. |
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