Cell Death Antibodies 1
Anti-CCAR1 Antibody (CAB13595)
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- SKU:
- CAB13595
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-CCAR1 Antibody |
| Antibody SKU: | CAB13595 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Mouse brain, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQF GGQK NPPW ATQF TATA VSQP AALG VQQP SLLG ASPT IYTQ QTAL AAAG LTTQ TPAN YQLT QTAA LQQQ AAAA AAAL QQQY SQPQ QALY SVQQ QLQQ PQQT LLTQ PAVA LPTS LSLS TPQP TAQI TVSY PTPR SSQQ QTQP QKQR VFTG VVTK LHDT FGFV DEDV FFQL SAVK GKTP QVGD RVLV EATY NPNM PFKW |
| Gene ID: | 55749 |
| Uniprot: | Q8IX12 |
| Cellular Location: | Cytoplasm, perinuclear region |
| Calculated MW: | 131kDa/132kDa |
| Observed MW: | 132KDa |
| Synonyms: | CCAR1 |
| Background: |
| UniProt Protein Function: | CCAR1: Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Apoptosis; RNA splicing Chromosomal Location of Human Ortholog: 10q21.3 Cellular Component: nucleoplasm; perinuclear region of cytoplasm Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity Biological Process: nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; RNA splicing; gene expression; cell cycle |
| UniProt Code: | Q8IX12 |
| NCBI GenInfo Identifier: | 94707499 |
| NCBI Gene ID: | 55749 |
| NCBI Accession: | Q8IX12.2 |
| UniProt Secondary Accession: | Q8IX12,Q32NE3, Q5EBM3, Q5VUP6, Q6PIZ0, Q6X935, Q9H8N4 A0JLT7, A1L4P7, A8K9D4, B4DNP8, B4DRK8, |
| UniProt Related Accession: | Q8IX12 |
| Molecular Weight: | 1150 |
| NCBI Full Name: | Cell division cycle and apoptosis regulator protein 1 |
| NCBI Synonym Full Names: | cell division cycle and apoptosis regulator 1 |
| NCBI Official Symbol: | CCAR1 |
| NCBI Protein Information: | cell division cycle and apoptosis regulator protein 1; death inducer with SAP domain; cell cycle and apoptosis regulatory protein 1 |
| UniProt Protein Name: | Cell division cycle and apoptosis regulator protein 1 |
| UniProt Synonym Protein Names: | Cell cycle and apoptosis regulatory protein 1; CARP-1; Death inducer with SAP domain |
| Protein Family: | Cell division cycle and apoptosis regulator protein |
| UniProt Gene Name: | CCAR1 |
| UniProt Entry Name: | CCAR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CCAR1 antibody (CAB13595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using CCAR1 antibody (CAB13595). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CCAR1 antibody (CAB13595). Western blot was performed from the immunoprecipitate using CCAR1 antibody (CAB13595) at a dilition of 1:500. |
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