Epigenetics & Nuclear Signaling Antibodies 2
Anti-CBX4 Antibody (CAB6221)
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- SKU:
- CAB6221
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CBX4 Antibody |
| Antibody SKU: | CAB6221 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AVGL NLSH VRKR CLSE THGE REPC KKRL TARS ISTP TCLG GSPA AERP ADLP PAAA LPQP EVIL LDSD LDEP IDLR CVKT RSEA GEPP SSLQ VKPE TPAS AAVA VAAA AAPT TTAE KPPA EAQD EPAE SLSE FKPF FGNI IITD VTAN CLTV TFKE YVTV |
| Gene ID: | 8535 |
| Uniprot: | O00257 |
| Cellular Location: | Nucleus, Nucleus speckle |
| Calculated MW: | 31kDa/61kDa |
| Observed MW: | 85kDa |
| Synonyms: | CBX4, NBP16, PC2 |
| Background: |
| UniProt Protein Function: | CBX4: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; SUMO ligase; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: nucleoplasm; nucleus; PcG protein complex Molecular Function:enzyme binding; protein binding; SUMO binding; SUMO ligase activity; transcription corepressor activity Biological Process: negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; protein sumoylation |
| UniProt Code: | O00257 |
| NCBI GenInfo Identifier: | 254763424 |
| NCBI Gene ID: | 8535 |
| NCBI Accession: | O00257.3 |
| UniProt Secondary Accession: | O00257,Q6TPI8, Q96C04, B1PJR7, |
| UniProt Related Accession: | O00257 |
| Molecular Weight: | 31,987 Da |
| NCBI Full Name: | E3 SUMO-protein ligase CBX4 |
| NCBI Synonym Full Names: | chromobox 4 |
| NCBI Official Symbol: | CBX4 |
| NCBI Official Synonym Symbols: | PC2; NBP16 |
| NCBI Protein Information: | E3 SUMO-protein ligase CBX4 |
| UniProt Protein Name: | E3 SUMO-protein ligase CBX4 |
| UniProt Synonym Protein Names: | Chromobox protein homolog 4; Polycomb 2 homolog; Pc2; hPc2 |
| Protein Family: | E3 SUMO-protein ligase |
| UniProt Gene Name: | CBX4 |
| UniProt Entry Name: | CBX4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CBX4 antibody (CAB6221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using CBX4 antibody (CAB6221) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CBX4 antibody (CAB6221). Western blot was performed from the immunoprecipitate using CBX4 antibody (CAB6221) at a dilition of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s. |
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