Antibody Name:	Anti-CBX1 Antibody
Antibody SKU:	CAB2247
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human CBX1 (NP_006798.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	OVCAR3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human CBX1 (NP_006798.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKK QNKK KVEE VLEE EEEE YVVE KVLD RRVV KGKV EYLL KWKG FSDE DNTW EPEE NLDC PDLI AEFL QSQK TAHE TDKS EGGK RKAD SDSE DKGE ESKP KKKK EESE KPRG FARG LEPE RIIG ATDS SGEL MFLM KWKN SDEA DLVP AKEA NVKC PQVV ISFY EERL TWHS YPSE DDDK KDDK N
Gene ID:	10951
Uniprot:	P83916
Cellular Location:	Nucleus
Calculated MW:	21kDa
Observed MW:	21kDa

Synonyms:

CBX1, CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta

Background:

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	CBX1: Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1 and SETDB1, SUV420H1 and SUV420H2. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Expressed in all adult and embryonic tissues.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: nucleoplasm; centric heterochromatin; male pronucleus; female pronucleus; spindle; nuclear heterochromatin; chromatin; chromosome, pericentric region; chromocenter Molecular Function:protein binding; protein homodimerization activity; enzyme binding; chromatin binding Biological Process: negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
UniProt Code:	P83916
NCBI GenInfo Identifier:	48428808
NCBI Gene ID:	5077
NCBI Accession:	P83916.1
UniProt Related Accession:	P23760
Molecular Weight:
NCBI Full Name:	Chromobox protein homolog 1
NCBI Synonym Full Names:	paired box 3
NCBI Official Symbol:	PAX3
NCBI Official Synonym Symbols:	WS1; WS3; CDHS; HUP2
NCBI Protein Information:	paired box protein Pax-3
UniProt Protein Name:	Chromobox protein homolog 1
UniProt Synonym Protein Names:	HP1Hsbeta; Heterochromatin protein 1 homolog beta; HP1 beta; Heterochromatin protein p25; M31; Modifier 1 protein; p25beta
Protein Family:	Chromobox protein
UniProt Gene Name:	CBX1
UniProt Entry Name:	CBX1_HUMAN

	Western blot analysis of extracts of OVCAR-3 cells, using CBX1 antibody (CAB2247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using CBX1 antibody (CAB2247) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using CBX1 antibody (CAB2247) at dilution of 1:100 (40x lens).