Name: Anti-CBLB Antibody (CAB2014)
Brand: Signal Transduction Antibodies 2
SKU: CAB2014
Availability: OutOfStock

Antibody Name:	Anti-CBLB Antibody
Antibody SKU:	CAB2014
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CBLB (NP_733762.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, 293T, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CBLB (NP_733762.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MANS MNGR NPGG RGGN PRKG RILG IIDA IQDA VGPP KQAA ADRR TVEK TWKL MDKV VRLC QNPK LQLK NSPP YILD ILPD TYQH LRLI LSKY DDNQ KLAQ LSEN EYFK IYID SLMK KSKR AIRL FKEG KERM YEEQ SQDR RNLT KLSL IFSH MLAE IKAI FPNG QFQG DNFR ITKA DAAE FWRK FFGD KTIV PWKV FRQC LHEV HQIS SGLE AMAL KSTI DLTC NDYI SVFE FDIF TRLF QPWG SILR NWNF LAVT HPGY MAFL TYDE VKAR LQKY STKP GSYI FRLS CTRL GQWA IGYV TGDG NILQ TIPH NKPL FQAL IDGS REGF YLYP DGRS YNPD LTGL CEPT PH
Gene ID:	868
Uniprot:	Q13191
Cellular Location:	Cytoplasm
Calculated MW:	86kDa/90kDa/109kDa
Observed MW:	109kDa

Synonyms:	CBLB, Cbl-b, Nbla00127, RNF56
Background:

UniProt Protein Function:	Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Calcium-binding; Ubiquitin ligase; Adaptor/scaffold; EC 6.3.2.-; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 3q13.11 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol Molecular Function:signal transducer activity; protein binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; ligase activity Biological Process: cell surface receptor linked signal transduction; T cell activation; positive regulation of protein catabolic process; negative regulation of alpha-beta T cell proliferation; negative regulation of T cell receptor signaling pathway; protein ubiquitination; immune response; NLS-bearing substrate import into nucleus; signal transduction; positive regulation of T cell anergy
UniProt Code:	Q13191
NCBI GenInfo Identifier:	88911265
NCBI Gene ID:	868
NCBI Accession:	Q13191.2
UniProt Secondary Accession:	Q13191,Q13192, Q13193, Q3LIC0, Q63Z43, Q8IVC5, A8K9S7 B7WNM4,
UniProt Related Accession:	Q13191
Molecular Weight:	Calculated MW: 86kDa/90kDa/109kDaObserved MW: 109kDa
NCBI Full Name:	E3 ubiquitin-protein ligase CBL-B
NCBI Synonym Full Names:	Cbl proto-oncogene B, E3 ubiquitin protein ligase
NCBI Official Symbol:	CBLB
NCBI Official Synonym Symbols:	Cbl-b; RNF56; Nbla00127
NCBI Protein Information:	E3 ubiquitin-protein ligase CBL-B; RING finger protein 56; SH3-binding protein CBL-B; signal transduction protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b
UniProt Protein Name:	E3 ubiquitin-protein ligase CBL-B
UniProt Synonym Protein Names:	Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56; SH3-binding protein CBL-B; Signal transduction protein CBL-B
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	CBLB
UniProt Entry Name:	CBLB_HUMAN

	Western blot analysis of extracts of various cell lines, using CBLB antibody (CAB2014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human liver damage using CBLB antibody (CAB2014) at dilution of 1:200 (40x lens).