Antibody Name:	Anti-CBFB Antibody
Antibody SKU:	CAB5305
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CBFB (NP_001746.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	THP-1, BT-474, 22Rv1, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CBFB (NP_001746.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPRV VPDQ RSKF ENEE FFRK LSRE CEIK YTGF RDRP HEER QARF QNAC RDGR SEIA FVAT GTNL SLQF FPAS WQGE QRQT PSRE YVDL EREA GKVY LKAP MILN GVCV IWKG WIDL QRLD GMGC LEFD EERA QQED ALAQ QAFE EARR RTRE FEDR DRSH REEM EVRV SQLL AVTG KKTT RP
Gene ID:	865
Uniprot:	Q13951
Cellular Location:	Nucleus
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

CBFB, PEBP2B

Background:

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CBFB: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. Belongs to the CBF-beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: membrane; nucleus Molecular Function:protein binding; DNA binding; transcription coactivator activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; lymphocyte differentiation; cell maturation; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13951
NCBI GenInfo Identifier:	2498753
NCBI Gene ID:	865
NCBI Accession:	Q13951.2
UniProt Secondary Accession:	Q13951,Q13124, Q9HCT2, A8K347,
UniProt Related Accession:	Q13951
Molecular Weight:	21,991 Da
NCBI Full Name:	Core-binding factor subunit beta
NCBI Synonym Full Names:	core-binding factor, beta subunit
NCBI Official Symbol:	CBFB
NCBI Official Synonym Symbols:	PEBP2B
NCBI Protein Information:	core-binding factor subunit beta; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; polyomavirus enhancer binding protein 2, beta subunit
UniProt Protein Name:	Core-binding factor subunit beta
UniProt Synonym Protein Names:	Polyomavirus enhancer-binding protein 2 beta subunit; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit
Protein Family:	Core-binding factor
UniProt Gene Name:	CBFB
UniProt Entry Name:	PEBB_HUMAN

	Western blot analysis of extracts of various cell lines, using CBFB antibody (CAB5305) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using CBFB Antibody (CAB5305) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CBFB Antibody (CAB5305) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using CBFB antibody (CAB5305). Blue: DAPI for nuclear staining.