Antibody Name:	Anti-CBFA2T2 Antibody
Antibody SKU:	CAB7033
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 325-604 of human CBFA2T2 (NP_005084.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Raji, HepG2, SW620, 22Rv1, Mouse brain, Mouse testis, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 325-604 of human CBFA2T2 (NP_005084.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLNG GYQD ELVD HRLT EREW ADEW KHLD HALN CIME MVEK TRRS MAVL RRCQ ESDR EELN YWKR RYNE NTEL RKTG TELV SRQH SPGS ADSL SNDS QREF NSRP GTGY VPVE FWKK TEEA VNKV KIQA MSEV QKAV AEAE QKAF EVIA TERA RMEQ TIAD VKRQ AAED AFLV INEQ EEST ENCW NCGR KASE TCSG CNIA RYCG SFCQ HKDW ERHH RLCG QNLH GQSP HGQG RPLL PVGR GSSA RSAD CSVP SPAL DKTS ATTS RSST PASV TAID TNGL
Gene ID:	9139
Uniprot:	O43439
Cellular Location:	Nucleus
Calculated MW:	28kDa/63kDa/65kDa/67kDa
Observed MW:	72kDa

Synonyms:

CBFA2T2, EHT, MTGR1, ZMYND3, p85

Background:

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

UniProt Protein Function:	CBFA2T2: May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Belongs to the CBFA2T family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 20q11 Cellular Component: nucleus Molecular Function:protein binding; metal ion binding; transcription corepressor activity; transcription factor activity Biological Process: epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
NCBI Summary:	In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	O43439
NCBI GenInfo Identifier:	34395613
NCBI Gene ID:	9139
NCBI Accession:	O43439.1
UniProt Secondary Accession:	O43439,Q5TGE4, Q5TGE5, Q5TGE6, Q5TGE7, Q8IWF3, Q96B06 Q96L00, Q9H436, Q9UJP8, B2RAE6, F8W6D7,
UniProt Related Accession:	O43439
Molecular Weight:	604
NCBI Full Name:	Protein CBFA2T2
NCBI Synonym Full Names:	core-binding factor, runt domain, alpha subunit 2; translocated to, 2
NCBI Official Symbol:	CBFA2T2
NCBI Official Synonym Symbols:	EHT; p85; MTGR1; ZMYND3
NCBI Protein Information:	protein CBFA2T2; MTG8-like protein; MTG8-related protein 1; ETO homolog on chromosome 20; ETO homologous on chromosome 20; myeloid translocation-related protein 1; myeloid translocation gene-related protein 1
UniProt Protein Name:	Protein CBFA2T2
UniProt Synonym Protein Names:	ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85
Protein Family:	Protein
UniProt Gene Name:	CBFA2T2
UniProt Entry Name:	MTG8R_HUMAN

	Western blot analysis of extracts of various cell lines, using CBFA2T2 antibody (CAB7033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using CBFA2T2 antibody (CAB7033).