Signal Transduction Antibodies 1

Anti-CAT Antibody (CAB11780)

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SKU:
CAB11780
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CAT Antibody
Antibody SKU:CAB11780
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human CAT (NP_001743.1).
Application:WB IP
Recommended Dilution:WB 1:1000 - 1:3000 IP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, HepG2, THP-1, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human CAT (NP_001743.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADS RDPA SDQM QHWK EQRA AQKA DVLT TGAG NPVG DKLN VITV GPRG PLLV QDVV FTDE MAHF DRER IPER VVHA KGAG AFGY FEVT HDIT KYSK AKVF EHIG KKTP IAVR FSTV AGES GSAD TVRD PRGF AVKF YTED GNWD LVGN NTPI FFIR DPIL FPSF IHSQ KRNP QTHL KDPD MVWD FWSL RPES LHQV SFLF SDRG IPDG HRHM NGYG SHTF KLVN A
Gene ID:847
Uniprot:P04040
Cellular Location:Peroxisome
Calculated MW:59kDa
Observed MW:60KDa
Synonyms:CAT, catalase, MGC138422, MGC138424
Background:This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.
UniProt Protein Function:Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - tryptophan; Endoplasmic reticulum; EC 1.11.1.6; Apoptosis; Energy Metabolism - methane; Mitochondrial; Hydrolase; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: peroxisomal membrane; Golgi apparatus; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol

Molecular Function:antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding

Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; ureteric bud development; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging

Disease: Acatalasemia

NCBI Summary:This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]
UniProt Code:P04040
NCBI GenInfo Identifier:115702
NCBI Gene ID:847
NCBI Accession:P04040.3
UniProt Related Accession:P04040
Molecular Weight:
NCBI Full Name:Catalase
NCBI Synonym Full Names:catalase
NCBI Official Symbol:CAT  
NCBI Protein Information:catalase
UniProt Protein Name:Catalase
Protein Family:Catalase
UniProt Gene Name:CAT  
UniProt Entry Name:CATA_HUMAN
Anti-CAT Antibody (CAB11780)Western blot analysis of extracts of various cell lines, using CAT antibody (CAB11780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-CAT Antibody (CAB11780)Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug CAT antibody (CAB11780). Western blot was performed from the immunoprecipitate using CAT antibody (CAB11780) at a dilition of 1:1000.
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