Cell Biology Antibodies 4

Anti-CAST Antibody (CAB13683)

(No reviews yet) Write a Review
SKU:
CAB13683
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CAST Antibody
Antibody SKU:CAB13683
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, NCI-H460, Mouse lung, Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDKD LDDA LDKL SDSL GQRQ PDPD ENKP MEDK VKEK AKAE HRDK LGER DDTI PPEY RHLL DDNG QDKP VKPP TKKS EDSK KPAD DQDP IDAL SGDL DSCP S
Gene ID:831
Uniprot:P20810
Cellular Location:
Calculated MW:63kDa/71- 84kDa
Observed MW:130kDa
Synonyms:CAST, BS-17, PLACK
Background:The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Inhibitor

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads

NCBI Summary:The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
UniProt Code:P20810
NCBI GenInfo Identifier:126302556
NCBI Gene ID:831
NCBI Accession:P20810.4
UniProt Related Accession:P20810
Molecular Weight:63kDa; 71-84kDa
NCBI Full Name:Calpastatin
NCBI Synonym Full Names:calpastatin
NCBI Official Symbol:CAST  
NCBI Official Synonym Symbols:BS-17; PLACK  
NCBI Protein Information:calpastatin
UniProt Protein Name:Calpastatin
UniProt Synonym Protein Names:Calpain inhibitor; Sperm BS-17 component
Protein Family:Calpastatin
UniProt Gene Name:CAST  
UniProt Entry Name:ICAL_HUMAN
Anti-CAST Antibody (CAB13683)Western blot analysis of extracts of various cell lines, using CAST antibody (CAB13683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CAST Antibody (CAB13683)Immunofluorescence analysis of U2OS cells using CAST antibody (CAB13683) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products