Cell Biology Antibodies 1
Anti-CAST Antibody (CAB0097)
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- SKU:
- CAB0097
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CAST Antibody |
| Antibody SKU: | CAB0097 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human CAST (NP_001177371.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human CAST (NP_001177371.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TIPP EYRH LLDD NGQD KPVK PPTK KSED SKKP ADDQ DPID ALSG DLDS CPST TETS QNTA KDKC KKAA SSSK APKN GGKA KDSA KTTE ETSK PKDD |
| Gene ID: | 831 |
| Uniprot: | P20810 |
| Cellular Location: | |
| Calculated MW: | 63kDa/71- 84kDa |
| Observed MW: | 124kDa |
| Synonyms: | CAST, BS-17, PLACK |
| Background: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 5q15 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads |
| NCBI Summary: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P20810 |
| NCBI GenInfo Identifier: | 126302556 |
| NCBI Gene ID: | 831 |
| NCBI Accession: | P20810.4 |
| UniProt Related Accession: | P20810 |
| Molecular Weight: | 63kDa; 71-84kDa |
| NCBI Full Name: | Calpastatin |
| NCBI Synonym Full Names: | calpastatin |
| NCBI Official Symbol: | CAST |
| NCBI Official Synonym Symbols: | BS-17; PLACK |
| NCBI Protein Information: | calpastatin |
| UniProt Protein Name: | Calpastatin |
| UniProt Synonym Protein Names: | Calpain inhibitor; Sperm BS-17 component |
| Protein Family: | Calpastatin |
| UniProt Gene Name: | CAST |
| UniProt Entry Name: | ICAL_HUMAN |
 | Western blot analysis of extracts of H460 cells, using CAST antibody (CAB0097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using CAST antibody (CAB0097) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using CAST antibody (CAB0097) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using CAST antibody (CAB0097) at dilution of 1:100 (40x lens). |
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