KO Validated Antibodies 1

Anti-Caspase-9 Antibody (CAB0281)[KO Validated]

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SKU:
CAB0281
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-Caspase-9 Antibody [KO Validated]
Antibody SKU:CAB0281
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human Caspase-9 (NP_001220.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human Caspase-9 (NP_001220.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDEA DRRL LRRC RLRL VEEL QVDQ LWDA LLSR ELFR PHMI EDIQ RAGS GSRR DQAR QLII DLET RGSQ ALPL FISC LEDT GQDM LASF LRTN RQAA KL
Gene ID:842
Uniprot:P55211
Cellular Location:
Calculated MW:17kDa/30kDa/36kDa/46kDa
Observed MW:36KDa/41KDa/46KDa
Synonyms:CASP9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase-9, casp9, Caspase 9
Background:This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.
UniProt Protein Details:

Protein type:Apoptosis; EC 3.4.22.62; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: apoptosome; cytosol

Molecular Function:cysteine-type endopeptidase activity; enzyme activator activity; peptidase activity; protein binding; protein kinase binding; SH3 domain binding

Biological Process: apoptosis; caspase activation via cytochrome c; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; platelet formation; response to DNA damage stimulus

NCBI Summary:This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:P55211
NCBI GenInfo Identifier:28558771
NCBI Gene ID:842
NCBI Accession:P55211.3
UniProt Secondary Accession:P55211,O95348, Q53Y70, Q5JRU9, Q5UGI1, Q92852, Q9BQ62 Q9UEQ3, Q9UIJ8, B4E1A3,
UniProt Related Accession:P55211
Molecular Weight:
NCBI Full Name:Caspase-9
NCBI Synonym Full Names:caspase 9
NCBI Official Symbol:CASP9  
NCBI Official Synonym Symbols:MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6  
NCBI Protein Information:caspase-9
UniProt Protein Name:Caspase-9
UniProt Synonym Protein Names:Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3
Protein Family:Caspase
UniProt Gene Name:CASP9  
Anti-Caspase-9 Antibody [KO Validated] (CAB0281)Western blot analysis of extracts of various cell lines, using Caspase-9 pAb (CAB0281) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37'C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Caspase-9 Antibody [KO Validated] (CAB0281)Western blot analysis of extracts from normal (control) and Caspase-9 knockout (KO) HeLa cells, using Caspase-9 antibody (CAB0281) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Caspase-9 Antibody [KO Validated] (CAB0281)Immunohistochemistry of paraffin-embedded human placenta using Caspase-9 antibody (CAB0281) at dilution of 1:100 (40x lens).
Anti-Caspase-9 Antibody [KO Validated] (CAB0281)Immunofluorescence analysis of C6 cells using Caspase-9 antibody (CAB0281) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-Caspase-9 Antibody [KO Validated] (CAB0281)Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody (CAB0281) at dilution of 1:100. Blue: DAPI for nuclear staining.
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