Cell Death Antibodies 1

Anti-Caspase-3 Antibody (CAB0214)

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SKU:
CAB0214
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-Caspase-3 Antibody
Antibody SKU:CAB0214
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Caspase-3 (NP_004337.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, 293T, NIH/3T3, mouse lung, mouse liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Caspase-3 (NP_004337.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRDV SKED HSKR SSFV CVLL SHGE EGII FGTN GPVD LKKI TNFF RGDR CRSL TGKP KLFI IQAC RGTE LDCG IETD SGVD DDMA CHKI PVEA DFLY AYST A
Gene ID:836
Uniprot:P42574
Cellular Location:Cytoplasm
Calculated MW:31kDa
Observed MW:15kDa/35kDa
Synonyms:CPP32, CPP32B, SCA-1, Active Caspase 3, CASP3, active Caspase-3, Caspase 3, Caspase-3 p12, caspase-3
Background:This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
UniProt Protein Function:CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.
UniProt Protein Details:

Protein type:Protease; EC 3.4.22.56; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol

Molecular Function:peptidase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis

NCBI Summary:This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:P42574
NCBI GenInfo Identifier:77416852
NCBI Gene ID:836
NCBI Accession:P42574.2
UniProt Secondary Accession:P42574,Q96AN1, Q96KP2, A8K5M2, D3DP53,
UniProt Related Accession:P42574
Molecular Weight:277
NCBI Full Name:Caspase-3
NCBI Synonym Full Names:caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol:CASP3  
NCBI Official Synonym Symbols:CPP32; SCA-1; CPP32B  
NCBI Protein Information:caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
UniProt Protein Name:Caspase-3
UniProt Synonym Protein Names:Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Protein Family:Caspase
UniProt Gene Name:CASP3  
UniProt Entry Name:CASP3_HUMAN
Anti-Caspase-3 Antibody (CAB0214)Western blot analysis of extracts of various cell lines, using Caspase-3 antibody (CAB0214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s
Anti-Caspase-3 Antibody (CAB0214)Western blot analysis of extracts of Jurkat cells, using Caspase-3 antibody (CAB0214) at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37'C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Caspase-3 Antibody (CAB0214)Immunohistochemistry of paraffin-embedded mouse lung using Caspase-3 Rabbit pAb (CAB0214) at dilution of 1:100 (40x lens).
Anti-Caspase-3 Antibody (CAB0214)Immunofluorescence analysis of L929 cells using Caspase-3 Rabbit pAb (CAB0214) at dilution of 1:100. Blue: DAPI for nuclear staining.
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