Developmental Biology
Anti-Caspase-14 Antibody (CAB6541)
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- SKU:
- CAB6541
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-Caspase-14 Antibody |
| Antibody SKU: | CAB6541 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human Caspase-14 (NP_036246.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human Caspase-14 (NP_036246.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSNP RSLE EEKY DMSG ARLA LILC VTKA REGS EEDL DALE HMFR QLRF ESTM KRDP TAEQ FQEE LEKF QQAI DSRE DPVS CAFV VLMA HGRE GFLK GEDG EMVK LENL FEAL NNKN CQAL RAKP KVYI IQAC RGEQ RDPG ETVG GDEI VMVI KDSP QTIP TYTD ALHV YSTV EGYI AYRH DQKG SCFI QTLV DVFT KRKG HILE LLTE VTRR MAEA ELVQ EGKA RKTN PEIQ STLR KRLY LQ |
| Gene ID: | 23581 |
| Uniprot: | P31944 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 27kDa |
| Observed MW: | 28-29kDa |
| Synonyms: | CASP14, ARCI12 |
| Background: | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. |
| UniProt Protein Function: | CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family. |
| UniProt Protein Details: | Protein type:EC 3.4.22.-; Protease Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: cytoplasm; keratin filament; nucleus Molecular Function:cysteine-type endopeptidase activity Biological Process: epidermis development; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; proteolysis |
| NCBI Summary: | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P31944 |
| NCBI GenInfo Identifier: | 12231007 |
| NCBI Gene ID: | 23581 |
| NCBI Accession: | P31944.2 |
| UniProt Secondary Accession: | P31944,O95823, Q3SYC9, |
| UniProt Related Accession: | P31944 |
| Molecular Weight: | 27,680 Da |
| NCBI Full Name: | Caspase-14 |
| NCBI Synonym Full Names: | caspase 14, apoptosis-related cysteine peptidase |
| NCBI Official Symbol: | CASP14 |
| NCBI Protein Information: | caspase-14; CASP-14; apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine protease |
| UniProt Protein Name: | Caspase-14 |
| Protein Family: | Caspase |
| UniProt Gene Name: | CASP14 |
| UniProt Entry Name: | CASPE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Caspase-14 antibody (CAB6541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of MCF7 cells using Caspase-14 antibody (CAB6541). Blue: DAPI for nuclear staining. |
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