Cell Biology Antibodies 16
Anti-Caspase-11 Antibody (CAB6495)
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- SKU:
- CAB6495
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Caspase-11 Antibody |
| Antibody SKU: | CAB6495 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-266 of mouse Caspase-4 (P70343). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | RAW264.7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 81-266 of mouse Caspase-4 (P70343). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 12363 |
| Uniprot: | |
| Cellular Location: | Endoplasmic reticulum membrane, Mitochondrion |
| Calculated MW: | 13kDa/18kDa/30kDa/36kDa/43kDa |
| Observed MW: | 30kDa |
| Synonyms: | Cas, ich-, Caspa, Caspl, ich-3, CASP-4, Casp11, CASP-11 |
| Background: | This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. |
 | Western blot analysis of extracts of RAW264.7 cells, using Caspase-11 antibody (CAB6495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 300s. |
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