Name: Anti-Caspase-10 Antibody (CAB0216)
Brand: Cell Death Antibodies 1
SKU: CAB0216
Availability: OutOfStock

Antibody Name:	Anti-Caspase-10 Antibody
Antibody SKU:	CAB0216
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-521 of human Caspase-10 (NP_116759.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-521 of human Caspase-10 (NP_116759.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VKTF LEAL PQES WQNK HAGS NGNR ATNG APSL VSRG MQGA SANT LNSE TSTK RAAV YRMN RNHR GLCV IVNN HSFT SLKD RQGT HKDA EILS HVFQ WLGF TVHI HNNV TKVE MEMV LQKQ KCNP AHAD GDCF VFCI LTHG RFGA VYSS DEAL IPIR EIMS HFTA LQCP RLAE KPKL FFIQ ACQG EEIQ PSVS IEAD ALNP EQAP TSLQ DSIP AEAD FLLG LATV PGYV SFRH VEEG SWYI QSLC NHLK KLVP RHED ILSI LTAV NDDV SRRV DKQG TKKQ MPQP AFTL RKKL VFPV PLDA LS
Gene ID:	843
Uniprot:	Q92851
Cellular Location:
Calculated MW:	28kDa/31kDa/51kDa/54kDa/58kDa
Observed MW:	72kDa

Synonyms:

CASP10, ALPS2, FLICE2, MCH4, caspase-10

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-\|-AMC and Asp-Glu-Val-Asp-\|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.22.63; Protease Chromosomal Location of Human Ortholog: 2q33-q34 Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol Molecular Function:protein binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia
NCBI Summary:	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]
UniProt Code:	Q92851
NCBI GenInfo Identifier:	12644463
NCBI Gene ID:	843
NCBI Accession:	Q92851.3
UniProt Secondary Accession:	Q92851,Q68HC0, Q6KF62, Q6KF63, Q8IUP5, Q8WYQ8, Q99845 Q9Y2U6, Q9Y2U7,
UniProt Related Accession:	Q92851
Molecular Weight:	521
NCBI Full Name:	Caspase-10
NCBI Synonym Full Names:	caspase 10, apoptosis-related cysteine peptidase
NCBI Official Symbol:	CASP10
NCBI Official Synonym Symbols:	MCH4; ALPS2; FLICE2
NCBI Protein Information:	caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
UniProt Protein Name:	Caspase-10
UniProt Synonym Protein Names:	Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4Cleaved into the following 2 chains:Caspase-10 subunit p23/17; Caspase-10 subunit p12
Protein Family:	Caspase
UniProt Gene Name:	CASP10
UniProt Entry Name:	CASPA_HUMAN

Western blot analysis of extracts of various cell lines, using Caspase-10 antibody (CAB0216). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies	ELISA Kits
Anti-Caspase-10 Antibody (CAB0169)	Human Caspase 10 ELISA Kit
CASP10 Antibody (PACO01812)	Human CASP10 (Caspase 10) ELISA Kit (HUES01816)
CASP10 Antibody (PACO08193)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)