Antibody Name:	Anti-Caspase-1 Antibody
Antibody SKU:	CAB0964
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase-1 (NP_150635.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase-1 (NP_150635.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADK VLKE KRKL FIRS MGEA PQAV QDNP AMPT SSGS EGNV KLCS LEEA QRIW KQKS AEIY PIMD KSSR TRLA LIIC NEEF DSIP RRTG AEVD ITGM TMLL QNLG YSVD VKKN LTAS DMTT ELEA FAHR PEHK TSDS TFLV FMSH GIRE GICG KKHS EQVP DILQ LNAI FNML NTKN CPSL KDKP KVII IQAC RGDS PGVV WFKD SVGV SGNL SLPT TEEF EDDA IKKA HIEK DFIA FCSS TPDN VSWR HPTM GSVF IGRL IEHM QEYA CSCD VEEI FRKV RFSF EQPD GRAQ MPTT ERVT LTRC FYLF PGH
Gene ID:	834
Uniprot:	P29466
Cellular Location:	Cytoplasm
Calculated MW:	10kDa/29kDa/35kDa/42kDa/45kDa
Observed MW:	48KDa/20-25KDa

Synonyms:

CASP1, ICE, IL1BC, P45, caspase-1, Caspase 1

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

UniProt Protein Function:	CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Protease; EC 3.4.22.36 Chromosomal Location of Human Ortholog: 11q23 Cellular Component: cytoplasm; extracellular region; cytosol Molecular Function:protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; signal transduction; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy
NCBI Summary:	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
UniProt Code:	P29466
NCBI GenInfo Identifier:	266321
NCBI Gene ID:	834
NCBI Accession:	P29466.1
UniProt Secondary Accession:	P29466,Q53EY6, Q6DMQ1, Q6GSS3, Q6PI75, Q9UCN3, B5MDZ1
UniProt Related Accession:	P29466
Molecular Weight:	10,417 Da
NCBI Full Name:	Caspase-1
NCBI Synonym Full Names:	caspase 1, apoptosis-related cysteine peptidase
NCBI Official Symbol:	CASP1
NCBI Official Synonym Symbols:	ICE; P45; IL1BC
NCBI Protein Information:	caspase-1; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
UniProt Protein Name:	Caspase-1
UniProt Synonym Protein Names:	Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10
UniProt Gene Name:	CASP1
UniProt Entry Name:	CASP1_HUMAN

	Western blot analysis of extracts of THP-1 cells, using Caspase-1 antibody (CAB0964) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37'C for overnight and LPS (1 ug/ml) at 37'C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
	Immunohistochemistry of paraffin-embedded human colon using Caspase-1 Rabbit pAb (CAB0964) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of Raw264.7 cells using Caspase-1 Polyclonal Antibody (CAB0964) at dilution of 1:200. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using Caspase-1 Rabbit pAb (CAB0964) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using Caspase-1 Rabbit pAb (CAB0964) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Caspase-1 Antibody (CAB16792)	Human Caspase-1 ELISA Kit
Anti-CASP1 Antibody (CAB18646)[KO Validated]	Human CASP1 (Caspase 1) CLIA Kit (HUES00016)
CASP1 Antibody (PACO00519)	Human CASP1 (Caspase 1) ELISA Kit (HUES01303)
Cleaved-CASP1 (D210) Antibody (PACO03145)	Human Caspase-1 (CASP1) ELISA Kit
Cleaved-CASP1 (M211) Antibody (PACO05449)	Caspase 1 Colorimetric Cell-Based ELISA Kit