Antibody Name:	Anti-CASP1 Antibody [KO Validated]
Antibody SKU:	CAB18646
Antibody Size:	20uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CASP1.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, THP-1, Mouse lung

Immunogen:	Recombinant protein of human CASP1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	834
Uniprot:	P29466
Cellular Location:	Cytoplasm
Calculated MW:	10kDa/29kDa/35kDa/42kDa/45kDa
Observed MW:	48kDa

	Western blot analysis of extracts from normal (control) and CASP1 knockout (KO) HeLa cells, using CASP1 antibody (18646) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using CASP1 antibody (CAB18646) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded Rat ovary using CASP1 antibody (CAB18646) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human liver cancer using CASP1 antibody (CAB18646) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using CASP1 antibody (CAB18646) at dilution of 1:100 (40x lens).

Synonyms:

CASP1, ICE, IL1BC, P45, caspase-1

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.

UniProt Protein Function:	CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Protease; EC 3.4.22.36 Chromosomal Location of Human Ortholog: 11q23 Cellular Component: cytoplasm; extracellular region; cytosol Molecular Function:protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; signal transduction; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy
NCBI Summary:	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
UniProt Code:	P29466
NCBI GenInfo Identifier:	266321
NCBI Gene ID:	834
NCBI Accession:	P29466.1
UniProt Secondary Accession:	P29466,Q53EY6, Q6DMQ1, Q6GSS3, Q6PI75, Q9UCN3, B5MDZ1
UniProt Related Accession:	P29466
Molecular Weight:	10,417 Da
NCBI Full Name:	Caspase-1
NCBI Synonym Full Names:	caspase 1, apoptosis-related cysteine peptidase
NCBI Official Symbol:	CASP1
NCBI Official Synonym Symbols:	ICE; P45; IL1BC
NCBI Protein Information:	caspase-1; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
UniProt Protein Name:	Caspase-1
UniProt Synonym Protein Names:	Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10
UniProt Gene Name:	CASP1
UniProt Entry Name:	CASP1_HUMAN

Antibodies	ELISA Kits
Anti-Caspase-1 Antibody (CAB0964)	Human Caspase-1 ELISA Kit
Anti-Caspase-1 Antibody (CAB16792)	Human CASP1 (Caspase 1) CLIA Kit (HUES00016)
CASP1 Antibody (PACO00519)	Human CASP1 (Caspase 1) ELISA Kit (HUES01303)
Cleaved-CASP1 (D210) Antibody (PACO03145)	Human Caspase-1 (CASP1) ELISA Kit
Cleaved-CASP1 (M211) Antibody (PACO05449)	Caspase 1 Colorimetric Cell-Based ELISA Kit