Antibody Name:	Anti-CASK Antibody
Antibody SKU:	CAB2501
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASK (NP_001119526.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	293T, U-251MG, BxPC-3, 22Rv1, NCI-H460, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASK (NP_001119526.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADD DVLF EDVY ELCE VIGK GPFS VVRR CINR ETGQ QFAV KIVD VAKF TSSP GLST EDLK REAS ICHM LKHP HIVE LLET YSSD GMLY MVFE FMDG ADLC FEIV KRAD AGFV YSEA VASH YMRQ ILEA LRYC HDNN IIHR DVKP HCVL LASK ENSA PVKL GGFG VAIQ LGES GLVA GGRV GTPH FMAP EVVK REPY GKPV DVWG CGVI LFIL LSGC LPFY GTKE RLFE GIIK GKYK MNPR QWSH ISES AKDL VRRM LMLD PAER ITVY EALN HPWL KERD RYAY KIHL PETV EQLR KFNA
Gene ID:	8573
Uniprot:	O14936
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein
Calculated MW:	59kDa/102kDa/103kDa/104kDa/105kDa
Observed MW:	115kDa

Synonyms:

CASK, CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8

Background:

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CASK: a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Adaptor/scaffold; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CASK family Chromosomal Location of Human Ortholog: Xp11.4 Cellular Component: presynaptic membrane; nuclear lamina; focal adhesion; nuclear matrix; basolateral plasma membrane; cytoplasm; plasma membrane; nucleolus; basement membrane; intercellular junction; cytosol; actin cytoskeleton Molecular Function:calmodulin binding; guanylate kinase activity; protein serine/threonine kinase activity; protein binding; neurexin binding; ATP binding Biological Process: extracellular matrix organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; negative regulation of cell-matrix adhesion; protein amino acid phosphorylation; nucleotide phosphorylation Disease: Mental Retardation And Microcephaly With Pontine And Cerebellar Hypoplasia; Fg Syndrome 4
NCBI Summary:	This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
UniProt Code:	O14936
NCBI GenInfo Identifier:	145559462
NCBI Gene ID:	8573
NCBI Accession:	O14936.3
UniProt Secondary Accession:	O14936,Q62915,
UniProt Related Accession:	O14936
Molecular Weight:	105/59kDa
NCBI Full Name:	Peripheral plasma membrane protein CASK
NCBI Synonym Full Names:	calcium/calmodulin dependent serine protein kinase
NCBI Official Symbol:	CASK
NCBI Official Synonym Symbols:	CMG; FGS4; LIN2; TNRC8; hCASK; CAGH39; CAMGUK; MICPCH; MRXSNA
NCBI Protein Information:	peripheral plasma membrane protein CASK
UniProt Protein Name:	Peripheral plasma membrane protein CASK
UniProt Synonym Protein Names:	Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog
Protein Family:	Caskin
UniProt Gene Name:	CASK
UniProt Entry Name:	CSKP_HUMAN

	Western blot analysis of extracts of various cell lines, using CASK antibody (CAB2501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using CASK antibody (CAB2501). Blue: DAPI for nuclear staining.