Metabolism Antibodies 1

Anti-CARS Antibody (CAB13676)

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SKU:
CAB13676
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CARS Antibody
Antibody SKU:CAB13676
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, BxPC-3, MCF7, Mouse liver, Mouse skeletal muscle, Rat spinal cord
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ILRA PVDI TGQF EKWG EEEA ELNK NFYD KKTA IHKA LCDN VDTR TVME EMRA LVSQ CNLY MAAR KAVR KRPN QALL ENIA LYLT HMLK IFGA VEED SSLG FPVG GPGT SLSL EATV MPYL QVLS EFRE GVRK IARE QKVP EILQ LSDA LRDN ILPE LGVR FEDH EGLP TVVK LVDR NTLL KERE EKRR VEEE KRKK KEEA ARRK QEQE AAKL AKMK IPPS EMFL SETD KYSK FDEN GLPT HDME GKEL SKGQ AKKL KKLF EAQE KLYK EYLQ MAQN GSFQ
Gene ID:833
Uniprot:P49589
Cellular Location:Cytoplasm
Calculated MW:82kDa/85kDa/94kDa
Observed MW:85kDa
Synonyms:CARS, CARS1, CYSRS, MGC:11246
Background:This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:CARS: a class 1 aminoacyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. Two alternatively spliced isoforms are described.
UniProt Protein Details:

Protein type:EC 6.1.1.16; Oncoprotein; Translation; Ligase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytosol

Molecular Function:protein binding; protein homodimerization activity; cysteine-tRNA ligase activity; metal ion binding; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; cysteinyl-tRNA aminoacylation; gene expression

NCBI Summary:This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
UniProt Code:P49589
NCBI GenInfo Identifier:20141641
NCBI Gene ID:833
NCBI Accession:P49589.3
UniProt Secondary Accession:P49589,Q53XI8, Q5HYE4, Q9HD24, Q9HD25,
UniProt Related Accession:P49589
Molecular Weight:748
NCBI Full Name:Cysteine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:cysteinyl-tRNA synthetase
NCBI Official Symbol:CARS  
NCBI Official Synonym Symbols:CARS1; CYSRS; MGC:11246  
NCBI Protein Information:cysteine--tRNA ligase, cytoplasmic; cysteine translase; cysteine tRNA ligase 1, cytoplasmic
UniProt Protein Name:Cysteine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Cysteinyl-tRNA synthetase; CysRS
Protein Family:CDP-archaeol synthase
UniProt Gene Name:CARS  
UniProt Entry Name:SYCC_HUMAN
Anti-CARS Antibody (CAB13676)Western blot analysis of extracts of various cell lines, using CARS antibody (CAB13676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-CARS Antibody (CAB13676)Immunohistochemistry of paraffin-embedded mouse lung using CARS Antibody (CAB13676) at dilution of 1:100 (40x lens).
Anti-CARS Antibody (CAB13676)Immunofluorescence analysis of U2OS cells using CARS antibody (CAB13676). Blue: DAPI for nuclear staining.
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