Immunology Antibodies 2

Anti-CARM1 Antibody (CAB2246)

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SKU:
CAB2246
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-CARM1 Antibody
Antibody SKU:CAB2246
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 409-608 of human CARM1 (NP_954592.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:22Rv1, A-431, Mouse spleen, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 409-608 of human CARM1 (NP_954592.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TEPL THWY QVRC LFQS PLFA KAGD TLSG TCLL IANK RQSY DISI VAQV DQTG SKSS NLLD LKNP FFRY TGTT PSPP PGSH YTSP SENM WNTG STYN LSSG MAVA GMPT AYDL SSVI ASGS SVGH NNLI PLAN TGIV NHTH SRMG SIMS TGIV QGSS GAQG SGGG STSA HYAV NSQF TMGG PAIS MASP MSIP TNTM HYGS
Gene ID:10498
Uniprot:Q86X55
Cellular Location:Cytoplasm, Nucleus
Calculated MW:41kDa/63kDa/65kDa
Observed MW:75kDa
Synonyms:CARM1, PRMT4
Background:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
UniProt Protein Function:CARM1: a protein arginine methyltransferase that methylates histones and other proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginine residues. A key epigenetic regulator of hematopoiesis affecting multiple lineages. Recruited to promoters upon gene activation together with histone acetyltransferases from P300 and p160 families, methylates histone H3 at R17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2 activation, acts synergically with P300 and either one of the p160 histone acetyltransferases SRC1, NCoA2 and SRC-3 or CTNNB1 to activate transcription. During myogenic transcriptional activation, acts together with SRC-3 as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53 transcriptional activation. Methylates P300, both at R 2142, which may loosen its interaction with NCoA2, and at R580 and R604 in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Methylates RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. Interacts with the C-terminus of NCoA2, SRC-3 and SRC1. Part of a complex consisting of CARM1, P300 and NCoA2. Interacts with FLII, TP53, myogenic factor MEF2, P300, TRIM24, CREBBP and CTNNB1. Identified in a complex containing CARM1, TRIM24 and NCoA2. Interacts with SRC3. Interacts with SNRPC. Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1. Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 K18 (H3K18ac) H3 K23 (H3K23ac) by EP300 and blocked by citrullination of H3 R17 (H3R17ci) by PADI4. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; Nuclear receptor co-regulator; Methyltransferase, protein arginine; EC 2.1.1.125

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:beta-catenin binding; histone methyltransferase activity; histone-arginine N-methyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein methyltransferase activity; protein-arginine N-methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; transcription coactivator activity

Biological Process: cellular lipid metabolic process; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; histone methylation; positive regulation of fat cell differentiation; regulation of estrogen receptor signaling pathway; regulation of transcription, DNA-dependent

NCBI Summary:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]
UniProt Code:Q86X55
NCBI GenInfo Identifier:308153622
NCBI Gene ID:10498
NCBI Accession:Q86X55.3
UniProt Secondary Accession:Q86X55,A6NN38,
UniProt Related Accession:Q86X55
Molecular Weight:41,885 Da
NCBI Full Name:Histone-arginine methyltransferase CARM1
NCBI Synonym Full Names:coactivator associated arginine methyltransferase 1
NCBI Official Symbol:CARM1  
NCBI Official Synonym Symbols:PRMT4  
NCBI Protein Information:histone-arginine methyltransferase CARM1
UniProt Protein Name:Histone-arginine methyltransferase CARM1
UniProt Synonym Protein Names:Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4
Protein Family:Probable histone-arginine methyltransferase
UniProt Gene Name:CARM1  
UniProt Entry Name:CARM1_HUMAN
Anti-CARM1 Antibody (CAB2246)Western blot analysis of extracts of various cell lines, using CARM1 antibody (CAB2246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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