Cell Death Antibodies 1

Anti-CARD8 Antibody (CAB0293)

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SKU:
CAB0293
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-CARD8 Antibody
Antibody SKU:CAB0293
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CARD8 (NP_003796.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:K-562, A673, ES-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CARD8 (NP_003796.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEAR DKQV LRSL RLEL GAEV LVEG LVLQ YLYQ EGIL TENH IQEI NAQT TGLR KTML LLDI LPSR GPKA FDTF LDSL QEFP WVRE KLKK AREE AMTD LPAG DRLT GIPS HILN SSPS DRQI NQLA QRLG PEWE PMVL SLGL SQTD IYRC KANH PHNV QSQV VEAF IRWR QRFG KQAT FQSL HNGL RAVE VDPS LLLH MLE
Gene ID:22900
Uniprot:Q9Y2G2
Cellular Location:Cytoplasm, Nucleus
Calculated MW:32kDa/44kDa/48kDa/55kDa/60kDa
Observed MW:60kDa
Synonyms:CARD8, CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN
Background:The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:protein binding; NACHT domain binding; protein homodimerization activity; caspase activator activity

Biological Process: caspase activation; positive regulation of caspase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion

NCBI Summary:The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
UniProt Code:Q9Y2G2
NCBI GenInfo Identifier:14424229
NCBI Gene ID:22900
NCBI Accession:Q9Y2G2.1
UniProt Secondary Accession:Q9Y2G2,Q6PGP8, Q96P82, B5KVR6, B7Z496, B7Z4A2, E9PEM7 G3XAM9,
UniProt Related Accession:Q9Y2G2
Molecular Weight:60,652 Da
NCBI Full Name:Caspase recruitment domain-containing protein 8
NCBI Synonym Full Names:caspase recruitment domain family, member 8
NCBI Official Symbol:CARD8  
NCBI Official Synonym Symbols:NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL  
NCBI Protein Information:caspase recruitment domain-containing protein 8; apoptotic protein NDPP1; CARD inhibitor of NF-kappaB-activating ligands; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
UniProt Protein Name:Caspase recruitment domain-containing protein 8
UniProt Synonym Protein Names:Apoptotic protein NDPP1; CARD-inhibitor of NF-kappa-B-activating ligand; CARDINAL; DACAR; Tumor up-regulated CARD-containing antagonist of CASP9; TUCAN
Protein Family:Caspase recruitment domain-containing protein
UniProt Gene Name:CARD8  
UniProt Entry Name:CARD8_HUMAN
Anti-CARD8 Antibody (CAB0293)Western blot analysis of extracts of various cell lines, using CARD8 antibody (CAB0293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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