Signal Transduction Antibodies 1
Anti-CARD19 Antibody (CAB13196)
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- SKU:
- CAB13196
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-CARD19 Antibody |
| Antibody SKU: | CAB13196 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CARD19 (NP_115686.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, A-549, HeLa, A-431, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CARD19 (NP_115686.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTDQ TYCD RLVQ DTPF LTGH GRLS EQQV DRII LQLN RYYP QILT NKEA EKFR NPKA SLRV RLCD LLSH LQRS GERD CQEF YRAL YIHA QPLH SRLP SRHA |
| Gene ID: | 84270 |
| Uniprot: | Q96LW7 |
| Cellular Location: | Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, Single-pass membrane protein |
| Calculated MW: | 20kDa/25kDa |
| Observed MW: | 20kDa |
| Synonyms: | CARD19, BinCARD, C9orf89 |
| Background: |
| UniProt Protein Function: | C9orf89 iso2: Plays a role in inhibiting the effects of BCL10-induced activation of NF-kappa-B. May inhibit the phosphorylation of BCL10 in a CARD-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 9q22.31 Cellular Component: endoplasmic reticulum membrane; mitochondrial membrane; integral to membrane; nucleus; cytosol Molecular Function:CARD domain binding Biological Process: negative regulation of I-kappaB kinase/NF-kappaB cascade |
| UniProt Code: | Q96LW7 |
| NCBI GenInfo Identifier: | 92087058 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_115686.3 |
| Molecular Weight: | 20kDa |
| NCBI Full Name: | caspase recruitment domain-containing protein 19 isoform 1 |
| UniProt Protein Name: | Bcl10-interacting CARD protein |
| Protein Family: | Caspase recruitment domain-containing protein |
| UniProt Gene Name: | C9orf89 |
| UniProt Entry Name: | BINCA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CARD19 antibody (CAB13196) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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