Cell Biology Antibodies 7

Anti-CARD16 Antibody (CAB16624)

(No reviews yet) Write a Review
SKU:
CAB16624

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CARD16 Antibody
Antibody SKU:CAB16624
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CARD16 (NP_001017534.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:mouse lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CARD16 (NP_001017534.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VMDK TRAL IDSV IPKG AQAC QICI TYIC EEDS YLAE TLGL SAAL QAVQ DNPA MPTC SSPE GRIK LCFL EDAQ RIWK QKLQ RCHV QNTI IKWS ERYT SGSF E
Gene ID:114769
Uniprot:Q5EG05
Cellular Location:
Calculated MW:10kDa/22kDa
Observed MW:28kDa
Synonyms:CARD16, COP, COP1, PSEUDO-ICE
Background:
UniProt Protein Function:CARD16: Caspase inhibitor. Acts as a regulator of procaspase- 1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro- inflammatory cytokine response. Also able to inhibit CASP1- mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. Down-regulated in patients suffering of Huntington disease. Homooligomer. Interacts with CASP1, CASP4, CARD8 and RIPK2. 2 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 11q22

UniProt Code:Q5EG05
NCBI GenInfo Identifier:74722547
NCBI Gene ID:114769
NCBI Accession:Q5EG05.1
UniProt Secondary Accession:Q5EG05,Q96RJ9,
UniProt Related Accession:Q5EG05
Molecular Weight:10,737 Da
NCBI Full Name:Caspase recruitment domain-containing protein 16
NCBI Synonym Full Names:caspase recruitment domain family member 16
NCBI Official Symbol:CARD16  
NCBI Official Synonym Symbols:COP; COP1; PSEUDO-ICE  
NCBI Protein Information:caspase recruitment domain-containing protein 16
UniProt Protein Name:Caspase recruitment domain-containing protein 16
UniProt Synonym Protein Names:Caspase recruitment domain-only protein 1; CARD-only protein 1; Caspase-1 inhibitor COP; Pseudo interleukin-1 beta converting enzyme; Pseudo-ICE; Pseudo-IL1B-converting enzyme
Protein Family:Caspase recruitment domain-containing protein
UniProt Gene Name:CARD16  
UniProt Entry Name:CAR16_HUMAN
Anti-CARD16 Antibody (CAB16624)Western blot analysis of extracts of mouse lung, using CARD16 antibody (CAB16624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

Additional Information

Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
View AllClose

Related Products