Cell Death Antibodies 1

Anti-CARD14 Antibody (CAB16569)

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SKU:
CAB16569
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-CARD14 Antibody
Antibody SKU:CAB16569
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human CARD14 (NP_001244899.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human CARD14 (NP_001244899.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SLSL HYSN ALQE KELA ASRC RSLQ EELY LLKQ ELQR ANMV SSCE LELQ EQSL RTAS DQES GDEE LNRL KEEN EKLR SLTF SLAE KDIL EQSL DEAR GSRQ ELVE RIHS LRER AVAA ERQR EQYW EEKE QTLL QFQK SKMA CQLY REKV NALQ AQVC ELQK ERDQ AYSA RDSA QREI SQSL VEKD SLRR QVFE LTDQ VCEL RTQL RQLQ AEPP GVLK QEAR TREP CPRE KQRL VRMH AICP RDDS DCSL VSST ESQL LSDL SATS SREL VDSF RSSS PAPP SQQS LYKR VAED FGEE PWSF S
Gene ID:79092
Uniprot:Q9BXL6
Cellular Location:Cytoplasm, Cytoplasm
Calculated MW:48kDa/83kDa/113kDa
Observed MW:113kDa
Synonyms:CARD14, BIMP2, CARMA2, PRP, PSORS2, PSS1
Background:This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:CARD14: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; plasma membrane

Molecular Function:CARD domain binding

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis; activation of NF-kappaB transcription factor

Disease: Psoriasis 2; Pityriasis Rubra Pilaris

NCBI Summary:This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
UniProt Code:Q9BXL6
NCBI GenInfo Identifier:384229040
NCBI Gene ID:79092
NCBI Accession:NP_001244899.1
UniProt Secondary Accession:Q9BXL6,Q9BVB5,
UniProt Related Accession:Q9BXL6
Molecular Weight:113,270 Da
NCBI Full Name:caspase recruitment domain-containing protein 14 isoform 3
NCBI Synonym Full Names:caspase recruitment domain family, member 14
NCBI Official Symbol:CARD14  
NCBI Official Synonym Symbols:PRP; PSS1; BIMP2; CARMA2; PSORS2  
NCBI Protein Information:caspase recruitment domain-containing protein 14; carma 2; card-maguk protein 2; CARD-containing MAGUK protein 2; bcl10-interacting maguk protein 2
UniProt Protein Name:Caspase recruitment domain-containing protein 14
UniProt Synonym Protein Names:CARD-containing MAGUK protein 2
UniProt Gene Name:CARD14  
UniProt Entry Name:CAR14_HUMAN
Anti-CARD14 Antibody (CAB16569)Western blot analysis of extracts of various cell lines, using CARD14 antibody (CAB16569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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