Antibody Name:	Anti-CARD11 Antibody
Antibody SKU:	CAB7140
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, Jurkat, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AKTL VQRL LNSG GAME FTIC KSDI VTRD EFLR RQKT ETII YSRE KNPN AFEC IAPA NIEA VAAK NKHC LLEA GIGC TRDL IKSN IYPI VLFI RVCE KNIK RFRK LLPR PETE EEFL RVCR LKEK ELEA LPCL YATV EPDM WGSV EELL RVVK DKIG EEQR KTIW VDED QL
Gene ID:	84433
Uniprot:	Q9BXL7
Cellular Location:	Cytoplasm, Membrane raft
Calculated MW:	133kDa
Observed MW:	133kDa

Synonyms:

CARD11, BENTA, BIMP3, CARMA1, IMD11, PPBL

Background:

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

UniProt Protein Function:	CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 7p22 Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft Molecular Function:guanylate kinase activity; protein binding; CARD domain binding Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy
NCBI Summary:	The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BXL7
NCBI GenInfo Identifier:	172046231
NCBI Gene ID:	84433
NCBI Accession:	Q9BXL7.3
UniProt Secondary Accession:	Q9BXL7,Q2NKN7, Q548H3, A4D1Z7,
UniProt Related Accession:	Q9BXL7
Molecular Weight:	133,284 Da
NCBI Full Name:	Caspase recruitment domain-containing protein 11
NCBI Synonym Full Names:	caspase recruitment domain family, member 11
NCBI Official Symbol:	CARD11
NCBI Official Synonym Symbols:	PPBL; BENTA; BIMP3; IMD11; CARMA1
NCBI Protein Information:	caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3
UniProt Protein Name:	Caspase recruitment domain-containing protein 11
UniProt Synonym Protein Names:	CARD-containing MAGUK protein 1; Carma 1
Protein Family:	Caspase recruitment domain-containing protein
UniProt Gene Name:	CARD11
UniProt Entry Name:	CAR11_HUMAN

	Western blot analysis of extracts of various cell lines, using CARD11 antibody (CAB7140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver damage using CARD11 antibody (CAB7140) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using CARD11 antibody (CAB7140). Blue: DAPI for nuclear staining.