Name: Anti-CAPN9 Antibody (CAB13782)
Brand: Signal Transduction Antibodies 1
SKU: CAB13782
Availability: OutOfStock

Antibody Name:	Anti-CAPN9 Antibody
Antibody SKU:	CAB13782
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 391-690 of human CAPN9 (NP_006606.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SGC-7901, HL-60, HT-29, Mouse stomach, Mouse small intestine, Rat stomach, Rat small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 391-690 of human CAPN9 (NP_006606.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QEEC SFLV ALMQ KDRR KLKR FGAN VLTI GYAI YECP DKDE HLNK DFFR YHAS RARS KTFI NLRE VSDR FKLP PGEY ILIP STFE PHQE ADFC LRIF SEKK AITR DMDG NVDI DLPE PPKP TPPD QETE EEQR FRAL FEQV AGED MEVT AEEL EYVL NAVL QKKK DIKF KKLS LISC KNII SLMD TSGN GKLE FDEF KVFW DKLK QWIN LFLR FDAD KSGT MSTY ELRT ALKA AGFQ LSSH LLQL IVLR YADE ELQL DFDD FLNC LVRL ENAS RVFQ ALST KNKE FIHL NINE FIHL TMNI
Gene ID:	10753
Uniprot:	O14815
Cellular Location:
Calculated MW:	76kDa/79kDa
Observed MW:	79kDa

Synonyms:

CAPN9, GC36, nCL-4, calpain-9

Background:

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CAPN9: Calcium-regulated non-lysosomal thiol-protease. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Calcium-binding; EC 3.4.22.-; Protease Chromosomal Location of Human Ortholog: 1q42.11-q42.3 Cellular Component: cytoplasm
NCBI Summary:	Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O14815
NCBI GenInfo Identifier:	32129432
NCBI Gene ID:	10753
NCBI Accession:	O14815.1
UniProt Secondary Accession:	O14815,Q9NS74, B1APS1, B1AQI0,
UniProt Related Accession:	O14815
Molecular Weight:	76,168 Da
NCBI Full Name:	Calpain-9
NCBI Synonym Full Names:	calpain 9
NCBI Official Symbol:	CAPN9
NCBI Official Synonym Symbols:	GC36; nCL-4
NCBI Protein Information:	calpain-9
UniProt Protein Name:	Calpain-9
UniProt Synonym Protein Names:	Digestive tract-specific calpain; New calpain 4; nCL-4; Protein CG36
Protein Family:	Calpain
UniProt Gene Name:	CAPN9
UniProt Entry Name:	CAN9_HUMAN

Western blot analysis of extracts of various cell lines, using CAPN9 antibody (CAB13782) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-CAPN9 Antibody (CAB17085)

CAPN9 Antibody (PACO02364)

CAPN9 Antibody (PACO08175)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)