Cell Biology Antibodies 4

Anti-CAPN6 Antibody (CAB13777)

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SKU:
CAB13777
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CAPN6 Antibody
Antibody SKU:CAB13777
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CAPN6 (NP_055104.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CAPN6 (NP_055104.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGPP LKLF KNQK YQEL KQEC IKDS RLFC DPTF LPEN DSLF YNRL LPGK VVWK RPQD ICDD PHLI VGNI SNHQ LTQG RLGH KPMV SAFS CLAV QESH WTKT IPNH KEQE WDPQ KTEK YAGI FHFR FWHF GEWT EVVI DDLL PTIN GDLV FSFS TSMN EFWN ALLE KAYA KLLG CYEA LDGL TITD IIVD FTGT LAET VDMQ KGRY TELV EEKY KLFG ELYK TFTK GGLI CCSI ESPN QEEQ EVET DWGL LKGH TYTM TDIR KIRL GERL VE
Gene ID:827
Uniprot:Q9Y6Q1
Cellular Location:Cytoplasm, cytoskeleton, perinuclear region, spindle
Calculated MW:74kDa
Observed MW:68kDa
Synonyms:CAPN6, CANPX, CAPNX, CalpM, DJ914P14.1, calpain-6
Background:Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis.
UniProt Protein Function:CAPN6: Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues. Belongs to the peptidase C2 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: spindle microtubule; perinuclear region of cytoplasm

Molecular Function:microtubule binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: proteolysis; microtubule bundle formation; regulation of cytoskeleton organization and biogenesis

NCBI Summary:Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]
UniProt Code:Q9Y6Q1
NCBI GenInfo Identifier:13959315
NCBI Gene ID:827
NCBI Accession:Q9Y6Q1.2
UniProt Related Accession:Q9Y6Q1
Molecular Weight:
NCBI Full Name:Calpain-6
NCBI Synonym Full Names:calpain 6
NCBI Official Symbol:CAPN6  
NCBI Official Synonym Symbols:CANPX; CAPNX; CalpM; DJ914P14.1  
NCBI Protein Information:calpain-6
UniProt Protein Name:Calpain-6
UniProt Synonym Protein Names:Calpain-like protease X-linked; Calpamodulin; CalpM
UniProt Gene Name:CAPN6  
UniProt Entry Name:CAN6_HUMAN
Anti-CAPN6 Antibody (CAB13777)Western blot analysis of extracts of rat heart, using CAPN6 antibody (CAB13777) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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