Cell Biology Antibodies 7
Anti-CAPN3 Antibody (CAB1893)
- SKU:
- CAB1893
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CAPN3 Antibody |
| Antibody SKU: | CAB1893 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-309 of human CAPN3 (NP_775111.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, THP-1, Mouse skeletal muscle, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-309 of human CAPN3 (NP_775111.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EEVE NTIS VDRP VKKK KTKP IIFV SDRA NSNK ELGV DQES EEGK GKTS PDKQ KQSP QPQP GSSD QESE EQQQ FRNI FKQI AGDD MEIC ADEL KKVL NTVV NKHK DLKT HGFT LESC RSMI ALMD TDGS GKLN LQEF HHLW NKIK AWQK IFKH YDTD QSGT INSY EMRN AVND AGFH LNNQ LYDI ITMR YADK HMNI DFDS FICC FVRL EGMF RAFH AFDK DGDG IIKL NVLE WLQL TMYA |
| Gene ID: | 825 |
| Uniprot: | P20807 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 18kDa/35kDa/84kDa/93kDa/94kDa |
| Observed MW: | 110kDa |
| Synonyms: | CAPN3, CANP3, CANPL3, LGMD2, LGMD2A, nCL-1, p94, calpain-3 |
| Background: | Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. |
| UniProt Protein Function: | CAPN3: Calcium-regulated non-lysosomal thiol-protease. Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A). LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset. Belongs to the peptidase C2 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell development/differentiation; Protease; Calcium-binding; EC 3.4.22.54 Chromosomal Location of Human Ortholog: 15q15.1 Cellular Component: protein complex; myofibril; T-tubule; cytoplasm; plasma membrane; intracellular; cytosol; Z disc; nucleus Molecular Function:peptidase activity; sodium ion binding; protein binding; signal transducer activity; structural constituent of muscle; titin binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; protein complex scaffold; catalytic activity; ligase regulator activity; cysteine-type peptidase activity Biological Process: response to muscle activity; muscle development; apoptosis; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of satellite cell activation involved in skeletal muscle regeneration; sarcomere organization; signal transduction; proteolysis; negative regulation of protein sumoylation; activation of NF-kappaB transcription factor; muscle maintenance; autolysis; myofibril assembly; regulation of catalytic activity; regulation of myoblast differentiation; protein complex assembly; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transcription, DNA-dependent; response to calcium ion; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of apoptosis Disease: Muscular Dystrophy, Limb-girdle, Type 2a |
| NCBI Summary: | Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20807 |
| NCBI GenInfo Identifier: | 1345664 |
| NCBI Gene ID: | 825 |
| NCBI Accession: | P20807.2 |
| UniProt Related Accession: | P20807 |
| Molecular Weight: | |
| NCBI Full Name: | Calpain-3 |
| NCBI Synonym Full Names: | calpain 3 |
| NCBI Official Symbol: | CAPN3 |
| NCBI Official Synonym Symbols: | p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A; LGMDD4; LGMDR1 |
| NCBI Protein Information: | calpain-3 |
| UniProt Protein Name: | Calpain-3 |
| UniProt Synonym Protein Names: | Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; New calpain 1; nCL-1 |
| UniProt Gene Name: | CAPN3 |
| UniProt Entry Name: | CAN3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAPN3 antibody (CAB1893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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