KO Validated Antibodies 1

Anti-CAPN1 Antibody (CAB18006)[KO Validated]

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SKU:
CAB18006
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CAPN1 Antibody [KO Validated]
Antibody SKU:CAB18006
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KQRA RELG LGRH ENAI KYLG QDYE QLRV RCLQ SGTL FRDE AFPP VPQS LGYK DLGP NSSK TYGI KWKR PTEL LSNP QFIV DGAT RTDI CQGA LGDC WLLA AIAS LTLN DTLL HRVV PHGQ SFQN GYAG IFHF QLWQ FGEW VDVV VDDL LPIK DGKL VFVH SAEG NEFW SALL EKAY AKVN GSYE ALSG GSTS EGFE DFTG GVTE WYEL RKAP SDLY QIIL KALE RGSL LGCS IDIS SVLD MEAI TFKK LVKG HAYS VTGA KQVN YRGQ VVSL IRMR NPWG EVEW TGAW SDSS SEWN NVDP YERD QLRV KME
Gene ID:823
Uniprot:P07384
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:81kDa
Observed MW:75kDa
Anti-CAPN1 Antibody [KO Validated] (CAB18006)Western blot analysis of extracts from normal (control) and CAPN1 knockout (KO) HeLa cells, using CAPN1 antibody (CAB18006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:CAPN1, CANP, CANP1, CANPL1, SPG76, muCANP, muCL, calpain 1
Background:The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:CAPN1: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family.
UniProt Protein Details:

Protein type:EC 3.4.22.52; Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: focal adhesion; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function:protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; receptor catabolic process; positive regulation of cell proliferation; proteolysis

NCBI Summary:The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:P07384
NCBI GenInfo Identifier:115574
NCBI Gene ID:823
NCBI Accession:P07384.1
UniProt Secondary Accession:P07384,Q2TTR0, Q6DHV4,
UniProt Related Accession:P07384
Molecular Weight:
NCBI Full Name:Calpain-1 catalytic subunit
NCBI Synonym Full Names:calpain 1, (mu/I) large subunit
NCBI Official Symbol:CAPN1  
NCBI Official Synonym Symbols:CANP; muCL; CANP1; CANPL1; muCANP  
NCBI Protein Information:calpain-1 catalytic subunit; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein
UniProt Protein Name:Calpain-1 catalytic subunit
UniProt Synonym Protein Names:Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP
Protein Family:Calpain
UniProt Gene Name:CAPN1  
UniProt Entry Name:CAN1_HUMAN
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