Immunology Antibodies 2
Anti-CANX Antibody (CAB15631)
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- SKU:
- CAB15631
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CANX Antibody |
| Antibody SKU: | CAB15631 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 501-592 of human CANX (NP_001737.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 501-592 of human CANX (NP_001737.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FCCS GKKQ TSGM EYKK TDAP QPDV KEEE EEKE EEKD KGDE EEEG EEKL EEKQ KSDA EEDG GTVS QEEE DRKP KAEE DEIL NRSP RNRK PRRE |
| Gene ID: | 821 |
| Uniprot: | P27824 |
| Cellular Location: | Endoplasmic reticulum, Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein |
| Calculated MW: | 55kDa/67kDa/71kDa |
| Observed MW: | 90KDa |
| Synonyms: | CNX, IP90, P90, Calnexin, CANX, calnexin |
| Background: | This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. |
| UniProt Protein Function: | Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum.Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Calcium-binding; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q35 Cellular Component: dendrite cytoplasm; endoplasmic reticulum membrane; protein complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; dendritic spine; cell soma; membrane; axon; melanosome; ribosome Molecular Function:ionotropic glutamate receptor binding; protein binding; unfolded protein binding; apolipoprotein binding; calcium ion binding; carbohydrate binding; glycoprotein binding Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; synaptic vesicle endocytosis; protein secretion; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging |
| NCBI Summary: | This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27824 |
| NCBI GenInfo Identifier: | 543920 |
| NCBI Gene ID: | 821 |
| NCBI Accession: | P27824.2 |
| UniProt Secondary Accession: | P27824,B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3, |
| UniProt Related Accession: | P27824 |
| Molecular Weight: | 55,598 Da |
| NCBI Full Name: | Calnexin |
| NCBI Synonym Full Names: | calnexin |
| NCBI Official Symbol: | CANX |
| NCBI Official Synonym Symbols: | CNX; P90; IP90 |
| NCBI Protein Information: | calnexin; major histocompatibility complex class I antigen-binding protein p88 |
| UniProt Protein Name: | Calnexin |
| UniProt Synonym Protein Names: | IP90; Major histocompatibility complex class I antigen-binding protein p88; p90 |
| UniProt Gene Name: | CANX |
| UniProt Entry Name: | CALX_HUMAN |
 | Western blot analysis of extracts of SW480 cells, using CANX Rabbit pAb (CAB15631) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using CANX antibody (CAB15631) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using CANX antibody (CAB15631) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug CANX antibody (CAB15631). Western blot was performed from the immunoprecipitate using CANX antibody (CAB15631) at a dilition of 1:500. |
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