Cell Biology Antibodies 12

Anti-CAMKK2 Antibody (CAB9899)

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SKU:
CAB9899
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CAMKK2 Antibody
Antibody SKU:CAB9899
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human CAMKK2 (NP_757380.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:NCI-H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human CAMKK2 (NP_757380.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSC VSSQ PSSN RAAP QDEL GGRG SSSS ESQK PCEA LRGL SSLS IHLG MESF IVVT ECEP GCAV DLGL ARDR PLEA DGQE VPLD TSGS QARP HLSG RKLS LQER SQGG LAAG GSLD MNGR CICP SLPY SPVS SPQS SPRL
Gene ID:10645
Uniprot:Q96RR4
Cellular Location:Cell projection, Cytoplasm, Nucleus
Calculated MW:54kDa/58kDa/59kDa/61kDa/64kDa
Observed MW:65kDa
Synonyms:CAMKK2, CAMKK, CAMKKB
Background:The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases.
UniProt Protein Function:CAMKK2: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Seems to be involved in hippocampal activation of CREB1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Autophagy; EC 2.7.11.17; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; CAMKK family; Meta subfamily

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: cell projection; cytoplasm; intracellular; nucleus

Molecular Function:calmodulin binding; calmodulin-dependent protein kinase activity; protein-tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; calcium-mediated signaling; MAPKKK cascade; regulation of protein kinase activity; protein amino acid phosphorylation

NCBI Summary:The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012]
UniProt Code:Q96RR4
NCBI GenInfo Identifier:317373374
NCBI Gene ID:10645
NCBI Accession:Q96RR4.2
UniProt Secondary Accession:Q96RR4,O94883, Q8IUG2, Q8IUG3, Q8N3I4, Q8WY03, Q8WY04 Q8WY05, Q8WY06, Q96RP1, Q96RP2, A8K7Q7,
UniProt Related Accession:Q96RR4
Molecular Weight:
NCBI Full Name:Calcium/calmodulin-dependent protein kinase kinase 2
NCBI Synonym Full Names:calcium/calmodulin-dependent protein kinase kinase 2, beta
NCBI Official Symbol:CAMKK2  
NCBI Official Synonym Symbols:CAMKK; CAMKKB  
NCBI Protein Information:calcium/calmodulin-dependent protein kinase kinase 2; caM-KK 2; caM-KK beta; CAMKK beta protein; caM-kinase kinase 2; caM-kinase kinase beta; calcium/calmodulin-dependent protein kinase beta
UniProt Protein Name:Calcium/calmodulin-dependent protein kinase kinase 2
UniProt Synonym Protein Names:Calcium/calmodulin-dependent protein kinase kinase beta; CaM-KK beta; CaM-kinase kinase beta; CaMKK beta
Protein Family:Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:CAMKK2  
UniProt Entry Name:KKCC2_HUMAN
Anti-CAMKK2 Polyclonal Antibody (CAB9899)Western blot analysis of extracts of H460 cells, using CAMKK2 antibody (CAB9899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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