Name: Anti-CAMK2B Antibody (CAB2508)
Brand: Developmental Biology
SKU: CAB2508
Availability: OutOfStock

Antibody Name:	Anti-CAMK2B Antibody
Antibody SKU:	CAB2508
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CAMK2B (NP_742078.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, PC-12, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CAMK2B (NP_742078.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATT VTCT RFTD EYQL YEDI GKGA FSVV RRCV KLCT GHEY AAKI INTK KLSA RDHQ KLER EARI CRLL KHSN IVRL HDSI SEEG FHYL VFDL VTGG ELFE DIVA REYY SEAD ASHC IQQI LEAV LHCH QMGV VHRD LKPE NLLL ASKC KGAA VKLA DFGL AIEV QGDQ QAWF GFAG TPGY LSPE VLRK EAYG KPVD IWAC GVIL YILL VGYP PFWD EDQH KLYQ QIKA GAYD FPSP EWDT VTPE AKNL INQM LTIN PAKR ITAH EALK HPWV CQRS TVAS MMHR QETV ECLK KFNA RRKL
Gene ID:	816
Uniprot:	Q13554
Cellular Location:	Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	50-60kDa/72kDa
Observed MW:	51kDa

Synonyms:

CAMK2B, CAM2, CAMK2, CAMKB

Background:

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CAMK2B: a widely expressed protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group. Functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. Involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban, an endogenous inhibitor of SERCA2. The holoenzyme is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2. Interacts with MPDZ. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle and induced during exercise. Eight isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:	Protein type:EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family Chromosomal Location of Human Ortholog: 7p14.3-p14.1 Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; plasma membrane; microtubule organizing center; spindle midzone; cytosol Molecular Function:calmodulin binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; actin binding; ATP binding Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of skeletal muscle adaptation; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; regulation of calcium ion transport; signal transduction; protein amino acid phosphorylation; regulation of synaptic transmission, cholinergic; inhibitory G-protein coupled receptor phosphorylation; synaptic transmission; peptidyl-serine phosphorylation; response to cadmium ion; calcium ion transport; regulation of synapse structural plasticity; neuromuscular process controlling balance; G1/S transition of mitotic cell cycle
NCBI Summary:	The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	Q13554
NCBI GenInfo Identifier:	334302890
NCBI Gene ID:	816
NCBI Accession:	Q13554.3
UniProt Secondary Accession:	Q13554,O95437, O95438, O95599, Q9UGH7, A4D2K0, A4D2K1 A4D2K2, A4D2K3, A4D2K4, A4D2K5, A4D2K6,
UniProt Related Accession:	Q13554
Molecular Weight:	664
NCBI Full Name:	Calcium/calmodulin-dependent protein kinase type II subunit beta
NCBI Synonym Full Names:	calcium/calmodulin-dependent protein kinase II beta
NCBI Official Symbol:	CAMK2B
NCBI Official Synonym Symbols:	CAM2; CAMK2; CAMKB
NCBI Protein Information:	calcium/calmodulin-dependent protein kinase type II subunit beta; caMK-II subunit beta; CaM-kinase II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase
UniProt Protein Name:	Calcium/calmodulin-dependent protein kinase type II subunit beta
Protein Family:	6-oxocamphor hydrolase
UniProt Gene Name:	CAMK2B
UniProt Entry Name:	KCC2B_HUMAN

Western blot analysis of extracts of various cell lines, using CAMK2B antibody (CAB2508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.