Name: Anti-Calpain-10 Antibody (CAB9865)
Brand: Cell Biology Antibodies 12
SKU: CAB9865
Availability: OutOfStock

Antibody Name:	Anti-Calpain-10 Antibody
Antibody SKU:	CAB9865
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CAPN10 (NP_075571.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:4000
Reactivity:	Human
Positive Samples:	HeLa, A375, HepG2, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CAPN10 (NP_075571.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRAG RGAT PARE LFRD AAFP AADS SLFC DLST PLAQ FRED ITWR RPQE ICAT PRLF PDDP REGQ VKQG LLGD CWFL CACA ALQK SRHL LDQV IPPG QPSW ADQE YRGS FTCR IWQF GRWV EVTT DDRL PCLA GRLC FSRC QRED VFWL PLLE KVYA KVHG SYEH LWAG QVAD ALVD LTGG LAER WNLK GVAG SGGQ QDRP GRWE HRTC RQLL HLKD QCLI SCCV LSPR AG
Gene ID:	11132
Uniprot:	Q9HC96
Cellular Location:
Calculated MW:	15kDa/29kDa/49kDa/57kDa/60kDa/74kDa
Observed MW:	68kDa

Synonyms:

CAPN10, CANP10, NIDDM1

Background:

Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene.

UniProt Protein Function:	CAPN10: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1). It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the peptidase C2 family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.22.-; Apoptosis; Mitochondrial; Protease Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: mitochondrion; cell; plasma membrane; cytosol Molecular Function:SNARE binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity Biological Process: positive regulation of glucose import; cellular response to insulin stimulus; positive regulation of intracellular transport; actin cytoskeleton reorganization; positive regulation of insulin secretion; proteolysis; cell structure disassembly during apoptosis Disease: Diabetes Mellitus, Noninsulin-dependent, 1
NCBI Summary:	Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]
UniProt Code:	Q9HC96
NCBI GenInfo Identifier:	13186302
NCBI Gene ID:	11132
NCBI Accession:	NP_075571.1
UniProt Secondary Accession:	Q9HC96,Q4ZFV1, Q8NCD4, Q96IG4, Q96JI2, Q9HC89, Q9HC90 Q9HC91, Q9HC92, Q9HC93, Q9HC94, A8MVS7,
UniProt Related Accession:	Q9HC96
Molecular Weight:	15,275 Da
NCBI Full Name:	calpain-10 isoform a
NCBI Synonym Full Names:	calpain 10
NCBI Official Symbol:	CAPN10
NCBI Official Synonym Symbols:	CANP10; NIDDM1
NCBI Protein Information:	calpain-10
UniProt Protein Name:	Calpain-10
UniProt Synonym Protein Names:	Calcium-activated neutral proteinase 10; CANP 10
Protein Family:	Calpain
UniProt Gene Name:	CAPN10
UniProt Entry Name:	CAN10_HUMAN

Anti-Calpain-10 Polyclonal Antibody (CAB9865)

Western blot analysis of extracts of various cell lines, using CAPN10 antibody (CAB9865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-CAPN10 Antibody (CAB14108)

CAPN10 Antibody (PACO02398)

CAPN10 Antibody (PACO08169)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)