Name: Anti-CALCOCO1 Antibody (CAB7987)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7987
Availability: OutOfStock

Antibody Name:	CALCOCO1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7987
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 462-691 of human CALCOCO1 (NP_065949.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HeLa, 293T, MCF7, A-549, Mouse lung, Mouse heart, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 462-691 of human CALCOCO1 (NP_065949.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVQL SESK RELT ELRS ALRV LQKE KEQL QEEK QELL EYMR KLEA RLEK VADE KWNE DATT EDEE AAVG LSCP AALT DSED ESPE DMRL PPYG LCER GDPG SSPA GPRE ASPL VVIS QPAP ISPH LSGP AEDS SSDS EAED EKSV LMAA VQSG GEEA NLLL PELG SAFY DMAS GFTV GTLS ETST GGPA TPTW KECP ICKE RFPA ESDK DALE DHMD GHFF FSTQ DPFT FE
Gene ID:	57658
Uniprot:	Q9P1Z2
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	67kDa/70kDa/77kDa
Observed MW:	77kDa

Synonyms:	CALCOCO1, Cocoa, PP13275, calphoglin
Background:

UniProt Protein Function:	CALCOCO1: Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1- mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. Belongs to the CALCOCO family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleus Molecular Function:protein C-terminus binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; sequence-specific DNA binding; transcription coactivator activity; transcription cofactor activity; beta-catenin binding; chromatin binding Biological Process: steroid hormone receptor signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; signal transduction
UniProt Code:	Q9P1Z2
NCBI GenInfo Identifier:	160017736
NCBI Gene ID:	57658
NCBI Accession:	Q9P1Z2.2
UniProt Secondary Accession:	Q9P1Z2,Q6FI59, Q71RC3, Q86WF8, Q96JU3, Q9H090, B3KVA8
UniProt Related Accession:	Q9P1Z2
Molecular Weight:	67,542 Da
NCBI Full Name:	Calcium-binding and coiled-coil domain-containing protein 1
NCBI Synonym Full Names:	calcium binding and coiled-coil domain 1
NCBI Official Symbol:	CALCOCO1
NCBI Official Synonym Symbols:	Cocoa; PP13275; calphoglin
NCBI Protein Information:	calcium-binding and coiled-coil domain-containing protein 1; coiled-coil coactivator protein; coiled-coil leucine zipper coactivator 1; inorganic pyrophosphatase activator; sarcoma antigen NY-SAR-3
UniProt Protein Name:	Calcium-binding and coiled-coil domain-containing protein 1
UniProt Synonym Protein Names:	Calphoglin; Coiled-coil coactivator protein; Sarcoma antigen NY-SAR-3
Protein Family:	Calcium-binding and coiled-coil domain-containing protein
UniProt Gene Name:	CALCOCO1
UniProt Entry Name:	CACO1_HUMAN

	Western blot analysis of extracts of various cell lines, using CALCOCO1 antibody (CAB7987) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using CALCOCO1 antibody (CAB7987) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using CALCOCO1 antibody (CAB7987). Blue: DAPI for nuclear staining.