Antibody Name:	Anti-CAD protein Antibody
Antibody SKU:	CAB8344
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, HepG2, HeLa, HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LERL GPGK GEVR PELG SRQD VEAL WENM AVID CFAS DHAP HTLE EKCG SRPP PGFP GLET MLPL LLTA VSEG RLSL DDLL QRLH HNPR RIFH LPPQ EDTY VEVD LEHE WTIP SHMP FSKA HWTP FEGQ KVKG TVRR VVLR GEVA YIDG QVLV PPGY GQDV RKWP QGAV PQLP PSAP ATSE MTTT PERP RRGI PGLP DGRF HLPP RIHR ASDP GLPA EEPK EKSS RKVA EPEL MGTP DGTC YPPP PVPR QAS
Gene ID:	790
Uniprot:	P27708
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	242kDa
Observed MW:	243kDa

Synonyms:

CAD, CDG1Z, EIEE50, GATD4

Background:

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.
UniProt Protein Details:	Protein type:EC 3.5.2.3; EC 6.3.5.5; Nucleotide Metabolism - pyrimidine; Amino Acid Metabolism - alanine, aspartate and glutamate; Ligase; Hydrolase; Transferase; EC 2.1.3.2 Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: cell projection; cell soma; cytosol; membrane; nuclear matrix; nucleoplasm; nucleus; terminal button Molecular Function:aspartate carbamoyltransferase activity; ATP binding; carbamoyl-phosphate synthase (ammonia) activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; dihydroorotase activity; enzyme binding; identical protein binding; protein kinase activity; zinc ion binding Biological Process: 'de novo' pyrimidine base biosynthetic process; arginine biosynthetic process; drug metabolic process; glutamine metabolic process; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; pyrimidine nucleoside biosynthetic process; urea cycle Disease: Congenital Disorder Of Glycosylation, Type Iz
NCBI Summary:	The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
UniProt Code:	P27708
NCBI GenInfo Identifier:	50403731
NCBI Gene ID:	790
NCBI Accession:	P27708.3
UniProt Secondary Accession:	P27708,Q6P0Q0, Q96CK3, D6W552,
UniProt Related Accession:	P27708
Molecular Weight:	242,984 Da
NCBI Full Name:	CAD protein
NCBI Synonym Full Names:	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
NCBI Official Symbol:	CAD
NCBI Official Synonym Symbols:	CDG1Z
NCBI Protein Information:	CAD protein
UniProt Protein Name:	CAD protein
Protein Family:	Cadherin
UniProt Gene Name:	CAD
UniProt Entry Name:	PYR1_HUMAN

	Western blot analysis of extracts of various cell lines, using CAD antibody (CAB8344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human tonsil using CAD antibody (CAB8344) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using CAD antibody (CAB8344) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using CAD antibody (CAB8344) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using CAD antibody (CAB8344) at dilution of 1:100. Blue: DAPI for nuclear staining.