KO Validated Antibodies 2

Anti-CACYBP Antibody (CAB20004)[KO Validated]

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SKU:
CAB20004
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CACYBP Antibody [KO Validated]
Antibody SKU:CAB20004
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CACYBP.
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human CACYBP.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:27101
Uniprot:Q9HB71
Cellular Location:Cytoplasm, Nucleus
Calculated MW:8kDa/21kDa/26kDa
Observed MW:26KDa
Anti-CACYBP Antibody [KO Validated] (CAB20004)Western blot analysis of extracts from normal (control) and CACYBP knockout (KO) 293T cells, using CACYBP antibody (CAB20004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:CACYBP, GIG5, PNAS-107, S100A6BP, SIP
Background:The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CACYBP: a nuclear/cytoplasmic protein involved in calcium-dependent ubiquitination and proteosomal degradation of target proteins. A possible molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). Interacts with proteins of the S100 family at physiological calcium concentrations. Component of some large E3 complexes and interacts directly with SIAH1, SIAH2 and SKP1A. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q24-q25

Cellular Component: nucleoplasm; neuron projection; nuclear envelope lumen; cytoplasm; beta-catenin destruction complex

Molecular Function:protein binding; protein homodimerization activity

Biological Process: positive regulation of DNA replication; cardiac muscle cell differentiation; aging

NCBI Summary:The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9HB71
NCBI GenInfo Identifier:46576651
NCBI Gene ID:27101
NCBI Accession:Q9HB71.2
UniProt Secondary Accession:Q9HB71,O60666, Q5R370, Q5R371, B2ZWH2, B3KSF1,
UniProt Related Accession:Q9HB71
Molecular Weight:21,228 Da
NCBI Full Name:Calcyclin-binding protein
NCBI Synonym Full Names:calcyclin binding protein
NCBI Official Symbol:CACYBP  
NCBI Official Synonym Symbols:SIP; GIG5; PNAS-107; S100A6BP  
NCBI Protein Information:calcyclin-binding protein; hCacyBP; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein
UniProt Protein Name:Calcyclin-binding protein
UniProt Synonym Protein Names:S100A6-binding protein; Siah-interacting protein
Protein Family:Calcyclin-binding protein
UniProt Gene Name:CACYBP  
UniProt Entry Name:CYBP_HUMAN
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