Name: Anti-CACNA2D4 Antibody (CAB12206)
Brand: Cell Biology Antibodies 2
SKU: CAB12206
Availability: OutOfStock

Antibody Name:	Anti-CACNA2D4 Antibody
Antibody SKU:	CAB12206
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 992-1115 of human CACNA2D4 (NP_758952.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HT-29, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 992-1115 of human CACNA2D4 (NP_758952.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AKSV FHHS HKHK KQDP LQPC DTEY PVFV YQPA IREA NGIV ECGP CQKV FVVQ QIPN SNLL LLVT DPTC DCSI FPPV LQEA TEVK YNAS VKCD RMRS QKLR RRPD SCHA FHPE ENAQ DCGG ASDT
Gene ID:	93589
Uniprot:	Q7Z3S7
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	4kDa/109kDa/121kDa/123kDa/127kDa/129kDa
Observed MW:	128kDa

Synonyms:

CACNA2D4, RCD4

Background:

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

UniProt Protein Function:	CACNA2D4: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Defects in CACNA2D4 are the cause of retinal cone dystrophy 4 (RCD4). RCD4 is characterized by minimal symptoms except for slowly progressive reduction in visual acuity. Belongs to the calcium channel subunit alpha-2/delta family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Channel, calcium; Membrane protein, integral Chromosomal Location of Human Ortholog: 12p13.33 Cellular Component: plasma membrane; voltage-gated calcium channel complex Molecular Function:voltage-gated calcium channel activity Biological Process: detection of light stimulus involved in visual perception Disease: Retinal Cone Dystrophy 4
NCBI Summary:	This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q7Z3S7
NCBI GenInfo Identifier:	296434419
NCBI Gene ID:	93589
NCBI Accession:	Q7Z3S7.2
UniProt Secondary Accession:	Q7Z3S7,Q7Z3S8, Q86XZ5, Q8IZS9,
UniProt Related Accession:	Q7Z3S7
Molecular Weight:	128kDa
NCBI Full Name:	Voltage-dependent calcium channel subunit alpha-2/delta-4
NCBI Synonym Full Names:	calcium voltage-gated channel auxiliary subunit alpha2delta 4
NCBI Official Symbol:	CACNA2D4
NCBI Official Synonym Symbols:	RCD4
NCBI Protein Information:	voltage-dependent calcium channel subunit alpha-2/delta-4
UniProt Protein Name:	Voltage-dependent calcium channel subunit alpha-2/delta-4
UniProt Synonym Protein Names:	Voltage-gated calcium channel subunit alpha-2/delta-4
Protein Family:	Voltage-dependent calcium channel
UniProt Gene Name:	CACNA2D4

Western blot analysis of extracts of various cell lines, using CACNA2D4 antibody (CAB12206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-CACNA2D4 Antibody (CAB12287)

CACNA2D4 Antibody (PACO00495)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)