Name: Anti-CACNA2D3 Antibody (CAB10315)
Brand: Cell Biology Antibodies 1
SKU: CAB10315
Availability: OutOfStock

Antibody Name:	Anti-CACNA2D3 Antibody
Antibody SKU:	CAB10315
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 900-1040 of human CACNA2D3 (NP_060868.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, HepG2, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 900-1040 of human CACNA2D3 (NP_060868.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LYDY QAMC RANK ESSD GAHG LLDP YNAF LSAV KWIM TELV LFLV EFNL CSWW HSDM TAKA QKLK QTLE PCDT EYPA FVSE RTIK ETTG NIAC EDCS KSFV IQQI PSSN LFMV VVDS SCLC ESVA PITM APIE IRYN ESLK C
Gene ID:	55799
Uniprot:	Q8IZS8
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	59kDa/112kDa/123kDa
Observed MW:	123kDa

Synonyms:

CACNA2D3, HSA272268

Background:

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

UniProt Protein Function:	CACNA2D3: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G). Belongs to the calcium channel subunit alpha-2/delta family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: plasma membrane; voltage-gated calcium channel complex Molecular Function:calcium channel activity; calcium channel regulator activity; metal ion binding; voltage-gated ion channel activity Biological Process: regulation of calcium ion transport
NCBI Summary:	This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8IZS8
NCBI GenInfo Identifier:	74723683
NCBI Gene ID:	55799
NCBI Accession:	Q8IZS8.1
UniProt Secondary Accession:	Q8IZS8,Q9NY16, Q9NY18, B2RPL6,
UniProt Related Accession:	Q8IZS8
Molecular Weight:	59,527 Da
NCBI Full Name:	Voltage-dependent calcium channel subunit alpha-2/delta-3
NCBI Synonym Full Names:	calcium voltage-gated channel auxiliary subunit alpha2delta 3
NCBI Official Symbol:	CACNA2D3
NCBI Official Synonym Symbols:	HSA272268
NCBI Protein Information:	voltage-dependent calcium channel subunit alpha-2/delta-3
UniProt Protein Name:	Voltage-dependent calcium channel subunit alpha-2/delta-3
UniProt Synonym Protein Names:	Voltage-gated calcium channel subunit alpha-2/delta-3
Protein Family:	Voltage-dependent calcium channel
UniProt Gene Name:	CACNA2D3
UniProt Entry Name:	CA2D3_HUMAN

Western blot analysis of extracts of various cell lines, using CACNA2D3 antibody (CAB10315) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

CACNA2D3 Antibody (PACO07170)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)