Antibody Name:	Anti-CA8 Antibody
Antibody SKU:	CAB7544
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CA8 (NP_004047.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	22Rv1, Mouse skeletal muscle, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human CA8 (NP_004047.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADL SFIE DTVA FPEK EEDE EEEE EGVE WGYE EGVE WGLV FPDA NGEY QSPI NLNS REAR YDPS LLDV RLSP NYVV CRDC EVTN DGHT IQVI LKSK SVLS GGPL PQGH EFEL YEVR FHWG RENQ RGSE HTVN FKAF PMEL HLIH WNST LFGS IDEA VGKP HGIA IIAL FVQI GKEH VGLK AVTE ILQD IQYK GKSK TIPC FNPN TLLP DPLL RDYW VYEG SLTI PPCS EGVT WILF RYPL TISQ LQIE EFRR LRTH VKGA ELVE GCDG ILGD NFRP TQPL SDRV IRAA FQ
Gene ID:	767
Uniprot:	P35219
Cellular Location:
Calculated MW:	32kDa
Observed MW:	33kDa

Synonyms:

CA8, CA-RP, CA-VIII, CALS, CAMRQ3, CARP

Background:

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Does not have a carbonic anhydrase catalytic activity.CautionAlthough it belongs to the alpha-carbonic anhydrase family, Arg-116 is present instead of the conserved His which is a zinc-binding residue. It is therefore expected that this protein lacks carbonic anhydrase activity.
NCBI Summary:	The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:	P35219
NCBI GenInfo Identifier:	461681
NCBI Gene ID:	767
NCBI Accession:	P35219.3
UniProt Secondary Accession:	P35219,Q32MY2, A8K0A5, B3KQZ7,
UniProt Related Accession:	P35219
Molecular Weight:	33 kDa
NCBI Full Name:	Carbonic anhydrase-related protein
NCBI Synonym Full Names:	carbonic anhydrase 8
NCBI Official Symbol:	CA8
NCBI Official Synonym Symbols:	CALS; CARP; CA-RP; CAMRQ3; CA-VIII
NCBI Protein Information:	carbonic anhydrase-related protein
UniProt Protein Name:	Carbonic anhydrase-related protein
UniProt Synonym Protein Names:	Carbonic anhydrase VIII; CA-VIII
Protein Family:	Carbonic anhydrase-related protein
UniProt Gene Name:	CA8

	Western blot analysis of extracts of various cell lines, using CA8 antibody (CAB7544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse cerebellum using CA8 antibody (CAB7544) at dilution of 1:100 (40x lens).